Abstract

A Spectroscopic Study of Dioxygen Reactivity with Non-Heme Metal Centers The goal of this project is to enhance the current understanding of the chemistry involved in the reactions of dioxygen (O2) with the non-heme bimettalic centers of hemerythrin and hemocyanin, two of the three known dioxygen-carrier proteins, using methods of time-resolved laser spectroscopy. Specifically femtosecond pump-probe, laser photolysis, time-resolved circular dichroism, and photoacoustic calorimetry experiments will be conduct to probe interactions between the metal centers and the active site environments of the target systems. It is our goal to determine the effect of protein structure and solvent conditions on the mechanism and rate of dioxygen binding and release. These experimental results will increase our limited understanding of how protein active site structure and composition control biologically relevant dioxygen binding reactions. In addition, these studies will have clinical impact in an important area: they will provide information on how non- heme metal centers reversibly bind dioxygen, and may facilitate the develop of new, more efficient, dioxygen carriers for blood substitutes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
2S06GM008111-29
Application #
6325659
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2000-06-05
Project End
2005-04-30
Budget Start
Budget End
Support Year
29
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Florida Agricultural and Mechanical University
Department
Type
DUNS #
City
Tallahassee
State
FL
Country
United States
Zip Code
32307

  Publications

Johnston, Jermaine G; Pollock, David M (2018) Circadian regulation of renal function. Free Radic Biol Med 119:93-107
Adams, Mark K; Lee, Seung-Ah; Belyaeva, Olga V et al. (2017) Characterization of human short chain dehydrogenase/reductase SDR16C family members related to retinol dehydrogenase 10. Chem Biol Interact 276:88-94
Mochona, Bereket; Jackson, Timothy; McCauley, DeCoria et al. (2016) Synthesis and Cytotoxic Evaluation of Pyrrole Hetarylazoles Containing Benzimidazole/Pyrazolone/1,3,4-Oxadiazole Motifs. J Heterocycl Chem 53:1871-1877
Engel, Krysta L; French, Sarah L; Viktorovskaya, Olga V et al. (2015) Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I. Mol Cell Biol 35:2321-31
Ayuk-Takem, Lambert; Amissah, Felix; Aguilar, Byron J et al. (2014) Inhibition of polyisoprenylated methylated protein methyl esterase by synthetic musks induces cell degeneration. Environ Toxicol 29:466-77
Chougule, Mahavir B; Patel, Apurva R; Patlolla, Ram et al. (2014) Epithelial transport of noscapine across cell monolayer and influence of absorption enhancers on in vitro permeation and bioavailability: implications for intestinal absorption. J Drug Target 22:498-508
Culpepper, Bonnie K; Webb, William M; Bonvallet, Paul P et al. (2014) Tunable delivery of bioactive peptides from hydroxyapatite biomaterials and allograft bone using variable-length polyglutamate domains. J Biomed Mater Res A 102:1008-16
Errahali, Younes J; Thomas, Leeshawn D; Keller 3rd, Thomas C S et al. (2013) Inhibition by new glucocorticoid antedrugs [16?, 17?-d] isoxazoline and [16?, 17?-d]-3'-hydroxy-iminoformyl isoxazoline derivatives of chemotaxis and CCL26, CCL11, IL-8, and RANTES secretion. J Interferon Cytokine Res 33:493-507
Stephenson, Adrienne P; Schneider, Jeffrey A; Nelson, Bryant C et al. (2013) Manganese-induced oxidative DNA damage in neuronal SH-SY5Y cells: attenuation of thymine base lesions by glutathione and N-acetylcysteine. Toxicol Lett 218:299-307
Godugu, Chandraiah; Patel, Apurva R; Doddapaneni, Ravi et al. (2013) Inhalation delivery of Telmisartan enhances intratumoral distribution of nanoparticles in lung cancer models. J Control Release 172:86-95

Showing the most recent 10 out of 40 publications