My long-range goal is to understand the roles of extracellular matrix (ECM) molecules, particularly fibronectin (FN) and laminin (LAM), in peripheral nervous system (PNS) development. This proposal focuses on potential roles for these molecules in neural crest migration and differentiation. It is known that ECM molecules can regulate cell shape, differentiation, and movement during embryogenesis. In the PNS, the adhesive glycoproteins FN an LAM are good candidates for mediating particular stages of neural crest migration and differentiation, due to their locations in embryos and interactions with crest cells in vitro. The first specific aim is to define the effects of these molecule on differentiation of subpopulations of neural crest cells. Avian crest cells well be cultured on substrata of FN, LAM, and defined proteolytic fragments of each, and the cultures evaluated for appearance of melanocytes and neurons. Second, mechanisms of crest migration on the above substrata will be examined, using timelapse video microscopy, computer analysis, and immunostaining of cytoskeletal components. Third, the optically-clear zebrafish embryo will be used to correlate in vivo behavior of crest cells with their interactions with specific ECM components. Video microscopy, scanning electron microscopy, and immunohistochemistry will be the predominant techniques used for these studies.

Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of New Mexico
Department
Type
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131
Bharadwaj, D; Mold, C; Markham, E et al. (2001) Serum amyloid P component binds to Fc gamma receptors and opsonizes particles for phagocytosis. J Immunol 166:6735-41
Romero, I R; Morris, C; Rodriguez, M et al. (1998) Inflammatory potential of C-reactive protein complexes compared to immune complexes. Clin Immunol Immunopathol 87:155-62
Tetzloff, S U; Bizzozero, O A (1998) Palmitoylation of proteolipid protein from rat brain myelin using endogenously generated 18O-fatty acids. J Biol Chem 273:279-85
Sanchez, P; Tetzloff, S U; Bizzozero, O A (1998) Veratridine-induced depolarization reduces the palmitoylation of brain and myelin glycerolipids. J Neurochem 70:1448-57
Bryant, J E; Hutchings, K G; Moyzis, R K et al. (1997) Measurement of telomeric DNA content in human tissues. Biotechniques 23:476-8, 480, 482, passim
Melendez, R F; Bizzozero, O A (1996) Palmitoylation of myelin P0 protein is independent of its synthesis and parallels that of phospholipids. J Peripher Nerv Syst 1:34-41
Mold, C; Gurule, C; Otero, D et al. (1996) Complement-dependent binding of C-reactive protein complexes to human erythrocyte CR1. Clin Immunol Immunopathol 81:153-60
Chapin, J E; Davis, L E; Kornfeld, M et al. (1995) Neurologic manifestations of intravascular lymphomatosis. Acta Neurol Scand 91:494-9
Smith, J P; Hicks, P S; Ortiz, L R et al. (1995) Quantitative measurement of muscle strength in the mouse. J Neurosci Methods 62:15-9
Varela, M F; Sansom, C E; Griffith, J K (1995) Mutational analysis and molecular modelling of an amino acid sequence motif conserved in antiporters but not symporters in a transporter superfamily. Mol Membr Biol 12:313-9

Showing the most recent 10 out of 18 publications