Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder characterized by the production of antinuclear antibodies to ribonucleoproteins and double-stranded DNA (dsDNA). Approximately 1.5 million Americans are affected by this illness, the majority being women. The etiology of SLE is unknown, but several viruses in particular the Epstein-Barr virus (EBV) have been implicated. There is an increase in the frequency of EBV infection observed in SLE patients compared to normal individuals. Epstein-Barr Nuclear Antigen-1 (EBNA-1) is a major antigen involved in viral latency that contains binding sites for viral DNA as well as chromosomes. We previously demonstrated that mice expressing EBNA-1 develop antibodies to dsDNA. The major objective of this proposal is to define the mechanism by which EBNA-1 exposure leads to the production of anti-dsDNA antibodies and whether these anti-dsDNA antibodies are pathogenic and deposit in the kidney. We will also determine whether or not EBNA-1 must complex with eukaryotic DNA in order to elicit an anti-DNA response or whether anti-EBNA-1 antibodies cross-react with dsDNA. Finally, we will address if there are strain specific differences in responsiveness to EBNA-1 and the development of antidsDNA antibodies and if this is mediated by TH1 orTH2 cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008168-30
Application #
8035942
Study Section
Minority Programs Review Committee (MPRC)
Project Start
Project End
Budget Start
2010-02-01
Budget End
2011-01-31
Support Year
30
Fiscal Year
2010
Total Cost
$191,997
Indirect Cost
Name
City College of New York
Department
Type
DUNS #
603503991
City
New York
State
NY
Country
United States
Zip Code
10031
Fried, Eric S; Li, Yue-Ming; Gilchrist, M Lane (2017) Phase Composition Control in Microsphere-Supported Biomembrane Systems. Langmuir 33:3028-3039
Gilchrist, M Lane; Ahn, Kwangwook; Li, Yue-Ming (2016) Imaging and Functional Analysis of ?-Secretase and Substrate in a Proteolipobead System with an Activity-Based Probe. Anal Chem 88:1303-11
Fried, Eric S; Luchan, Joshua; Gilchrist, M Lane (2016) Biodegradable, Tethered Lipid Bilayer-Microsphere Systems with Membrane-Integrated ?-Helical Peptide Anchors. Langmuir 32:3470-5
Banerjee, Shaibal; Sinha, Saikat; Pradhan, Padmanava et al. (2016) Regiospecifically Fluorinated Polycyclic Aromatic Hydrocarbons via Julia-Kocienski Olefination and Oxidative Photocyclization. Effect of Fluorine Atom Substitution on Molecular Shape. J Org Chem 81:3983-93
Beck, Cade; Singh, Tanya; Farooqi, Angela et al. (2016) Controlled microfluidics to examine growth-factor induced migration of neural progenitors in the Drosophila visual system. J Neurosci Methods 262:32-40
Salas-Ramirez, Kaliris Y; Bagnall, Ciara; Frias, Leslie et al. (2015) Doxorubicin and cyclophosphamide induce cognitive dysfunction and activate the ERK and AKT signaling pathways. Behav Brain Res 292:133-41
Thomson, Paul F; Parrish, Damon; Pradhan, Padmanava et al. (2015) Modular, Metal-Catalyzed Cycloisomerization Approach to Angularly Fused Polycyclic Aromatic Hydrocarbons and Their Oxidized Derivatives. J Org Chem 80:7435-46
Small, Chiyedza; Ramroop, Johnny; Otazo, Maria et al. (2014) An unexpected link between notch signaling and ROS in restricting the differentiation of hematopoietic progenitors in Drosophila. Genetics 197:471-83
Zhong, Lina; Tu, Raymond; Gilchrist, M Lane (2013) Tether-supported biomembranes with ?-helical peptide-based anchoring constructs. Langmuir 29:299-307
Guleyupoglu, Berkan; Schestatsky, Pedro; Edwards, Dylan et al. (2013) Classification of methods in transcranial electrical stimulation (tES) and evolving strategy from historical approaches to contemporary innovations. J Neurosci Methods 219:297-311

Showing the most recent 10 out of 94 publications