Abstract

Chromatin structure has been studied by many investigators to understand its role in the biological activity of DNA, namely, its role in gene expression. The focus has been on higher organisms and a great deal has been learned about the fundamental unit of chromatin, the nucleosome. This proposal involves an analysis of chromatin structure of a lower eukaryotic organism, the yeast Saccharomyces cerevisiae. There appear to be significant differences between the chromatin structure of higher and lower organisms. There are two major reasons for undertaking these studies. (1) Yeast is often used as a host for recombinant DNA studies. Optimal gene expression of foreign DNA will probably require a full understanding of yeast chromatin. (2) As a comparative study, differences in yeast and higher eukaryotic chromatin structure will be clearly defined by a number of physical and enzymatic methods. Reconstitution experiments using hybrid mixtures of histones from yeast and higher eukaryotes will provide information which will be used to assign particular changes in the chromatin structure to specific proteins (histones).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008192-11
Application #
3877543
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
San Jose State University
Department
Type
DUNS #
City
San Jose
State
CA
Country
United States
Zip Code
95112

  Publications

Saleh, Nidal; Moore 2nd, Barry; Srebro, Monika et al. (2015) Acid/base-triggered switching of circularly polarized luminescence and electronic circular dichroism in organic and organometallic helicenes. Chemistry 21:1673-81
Saleh, Nidal; Srebro, Monika; Reynaldo, Thibault et al. (2015) enantio-Enriched CPL-active helicene-bipyridine-rhenium complexes. Chem Commun (Camb) 51:3754-7
Zhang, Xiao-Peng; Chang, Victoria Y; Liu, Jian et al. (2015) Potential switchable circularly polarized luminescence from chiral cyclometalated platinum(II) complexes. Inorg Chem 54:143-52
Sundar, M Shyam; Talele, Harish R; Mande, Hemant M et al. (2014) Synthesis of enantiomerically pure helicene like bis-oxazines from atropisomeric 7,7'-dihydroxy BINOL: Preliminary measurements of the circularly polarized luminescence. Tetrahedron Lett 55:1760-1764
Sánchez-Carnerero, Esther M; Moreno, Florencio; Maroto, Beatriz L et al. (2014) Circularly polarized luminescence by visible-light absorption in a chiral O-BODIPY dye: unprecedented design of CPL organic molecules from achiral chromophores. J Am Chem Soc 136:3346-9
Shen, Chengshuo; Anger, Emmanuel; Srebro, Monika et al. (2014) Straightforward access to mono- and bis-cycloplatinated helicenes that display circularly polarized phosphorescence using crystallization resolution methods. Chem Sci 5:1915-1927
Tohgha, Urice; Deol, Kirandeep K; Porter, Ashlin G et al. (2013) Ligand induced circular dichroism and circularly polarized luminescence in CdSe quantum dots. ACS Nano 7:11094-102
Brunet, Ernesto; Jimenez, Laura; de Victoria-Rodriguez, Maria et al. (2013) The use of lanthanide luminescence as a reporter in the solid state: Desymmetrization of the prochiral layers of ýý-zirconium phosphate/phosphonate and circularly polarized luminescence. Microporous Mesoporous Mater 169:222-234
Bozoklu, Gulay; Gateau, Christelle; Imbert, Daniel et al. (2012) Metal-controlled diastereoselective self-assembly and circularly polarized luminescence of a chiral heptanuclear europium wheel. J Am Chem Soc 134:8372-5
Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A et al. (2012) Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28. Toxicon 59:241-8

Showing the most recent 10 out of 47 publications