Abstract

The primary objective of this project is to evaluate the capabilities of various micro-separation techniques (capillary electrophoresis, capillary electrochromatography, capillary HPLC as well as hybrid methods) for the analysis of biologically and pharmaceutically important samples. A protocol will be developed that allows testing of a broad range of solute classes from small molecules thoroughly macromolecules in order to determine the most suitable method(s) for solving a particular type of separation problem. Because samples related to biological, physiological or pharmaceutical processes are often limited in size or amount of material, the use of micro-separation techniques is highly desirable. The protocol described in this proposal should provide both a better fundamental understanding of micro-separation methods and their interrelationships as well as a practical guide to other scientists for selecting the most appropriate means for various types of biomolecule and pharmaceutical analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
2S06GM008192-20
Application #
6215756
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2000-09-01
Project End
2004-08-31
Budget Start
Budget End
Support Year
20
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
San Jose State University
Department
Type
DUNS #
City
San Jose
State
CA
Country
United States
Zip Code
95112

  Publications

Saleh, Nidal; Moore 2nd, Barry; Srebro, Monika et al. (2015) Acid/base-triggered switching of circularly polarized luminescence and electronic circular dichroism in organic and organometallic helicenes. Chemistry 21:1673-81
Saleh, Nidal; Srebro, Monika; Reynaldo, Thibault et al. (2015) enantio-Enriched CPL-active helicene-bipyridine-rhenium complexes. Chem Commun (Camb) 51:3754-7
Zhang, Xiao-Peng; Chang, Victoria Y; Liu, Jian et al. (2015) Potential switchable circularly polarized luminescence from chiral cyclometalated platinum(II) complexes. Inorg Chem 54:143-52
Sundar, M Shyam; Talele, Harish R; Mande, Hemant M et al. (2014) Synthesis of enantiomerically pure helicene like bis-oxazines from atropisomeric 7,7'-dihydroxy BINOL: Preliminary measurements of the circularly polarized luminescence. Tetrahedron Lett 55:1760-1764
Sánchez-Carnerero, Esther M; Moreno, Florencio; Maroto, Beatriz L et al. (2014) Circularly polarized luminescence by visible-light absorption in a chiral O-BODIPY dye: unprecedented design of CPL organic molecules from achiral chromophores. J Am Chem Soc 136:3346-9
Shen, Chengshuo; Anger, Emmanuel; Srebro, Monika et al. (2014) Straightforward access to mono- and bis-cycloplatinated helicenes that display circularly polarized phosphorescence using crystallization resolution methods. Chem Sci 5:1915-1927
Tohgha, Urice; Deol, Kirandeep K; Porter, Ashlin G et al. (2013) Ligand induced circular dichroism and circularly polarized luminescence in CdSe quantum dots. ACS Nano 7:11094-102
Brunet, Ernesto; Jimenez, Laura; de Victoria-Rodriguez, Maria et al. (2013) The use of lanthanide luminescence as a reporter in the solid state: Desymmetrization of the prochiral layers of ýý-zirconium phosphate/phosphonate and circularly polarized luminescence. Microporous Mesoporous Mater 169:222-234
Bozoklu, Gulay; Gateau, Christelle; Imbert, Daniel et al. (2012) Metal-controlled diastereoselective self-assembly and circularly polarized luminescence of a chiral heptanuclear europium wheel. J Am Chem Soc 134:8372-5
Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A et al. (2012) Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28. Toxicon 59:241-8

Showing the most recent 10 out of 47 publications