The objective of this research is to study the role of retinyl ester in the mammalian visual cycle. Specifically, we will conduct biochemical analyses to evaluate a retinyl ester hydrolase which catalyses the hydrolysis of retinyl esters in the retinal pigment epithelium. Earlier results have shown that this enzyme is specific for the physiologically important 11- cis isomer and it has a sufficiently high activity to release retinal as precursor of visual pigment chromophore. Many detailed biochemical properties of the enzyme remain to be explored. We propose to further examine the location of this hydrolase in the plasma membrane which is a possible site of the enzyme. We also plan to purify this enzyme and study its activity in its native membrane, in solution and in reconstituted membranes. Our investigation into the utilization of retinyl esters in the eye will provide important information relating to various ocular disorders. One MBRS graduate and one MBRS undergraduate student will participate in the study of the research of visual biochemistry of the eye. Student training will include designing and performing experiments, participation in laboratory meetings, presentation of research in laboratory journal club and at national meetings. Students will also participate int he preparation of research manuscripts and coauthor publication of experimental findings in refereed, scientific journals.

Project Start
1997-08-01
Project End
1998-07-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
18
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78249
Ye, Ruquan; Dong, Juncai; Wang, Luqing et al. (2018) Manganese deception on graphene and implications in catalysis. Carbon N Y 132:623-631
Liu, Lian; Zhang, Shao-Wu; Zhang, Yu-Chen et al. (2015) Decomposition of RNA methylome reveals co-methylation patterns induced by latent enzymatic regulators of the epitranscriptome. Mol Biosyst 11:262-74
Gonzalez-Fernandez, Federico; Betts-Obregon, Brandi; Yust, Brian et al. (2015) Interphotoreceptor retinoid-binding protein protects retinoids from photodegradation. Photochem Photobiol 91:371-8
Wooten, Marilyn; Karra, Sushma; Zhang, Maogen et al. (2014) On the direct electron transfer, sensing, and enzyme activity in the glucose oxidase/carbon nanotubes system. Anal Chem 86:752-7
Cantu-Valle, J; Ruiz-Zepeda, F; Voelkl, E et al. (2013) Determination of the surface morphology of gold-decahedra nanoparticles using an off-axis electron holography dual-lens imaging system. Micron 54-55:82-6
Obregon, Isidro D; Betts-Obregon, Brandi S; Yust, Brian et al. (2013) Effect of Silver Coating on Barium Titanium Oxide Nanoparticle Toxicity. Adv Mat Res 787:404-407
Casillas, Gilberto; Ponce, Arturo; Velázquez-Salazar, J Jesús et al. (2013) Direct observation of liquid-like behavior of a single Au grain boundary. Nanoscale 5:6333-7
Yehdego, Daniel T; Zhang, Boyu; Kodimala, Vikram K R et al. (2013) Secondary Structure Predictions for Long RNA Sequences Based on Inversion Excursions and MapReduce. IEEE Int Symp Parallel Distrib Process Workshops Phd Forum 2013:520-529
Pudasaini, Pushpa Raj; Ruiz-Zepeda, Francisco; Sharma, Manisha et al. (2013) High efficiency hybrid silicon nanopillar-polymer solar cells. ACS Appl Mater Interfaces 5:9620-7
Parvathaneni, Kalpana; Grigsby, Jeffery G; Betts, Brandi S et al. (2013) Estrogen-induced retinal endothelial cell proliferation: possible involvement of pigment epithelium-derived factor and phosphoinositide 3-kinase/mitogen-activated protein kinase pathways. J Ocul Pharmacol Ther 29:27-32

Showing the most recent 10 out of 99 publications