Mycotoxins are a class of compounds produced by certain strains of fungi or molds. These compounds may be toxic. Human health hazards and laboratory animals health hazards are possibly due to mycotoxin contaminated foods. The continuation project purposes are as follows: (1) To develop a method using supercritical fluid extraction (SFE) for extraction of aflatoxins from peanut butter and grains, (2) To continue to study cyclic voltammetry properties of mycotoxins - Aflatoxins B1, B2, G1, G2, aflatoxicol, and patulin using electrochemical reduction in various pH media, (3) To determine the number of electrons transferred and propose the possible organic mechanisms involved, and (4) To determine the percentage recoveries of spiked samples of aflatoxins. Undergraduate students knowledge will be enhanced by operating polarographic equipment HPLC instrument, and SFE equipment. Knowledge will also be gained with analytical skills, techniques and presentations.

Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Arkansas at Pine Bluff
Department
Type
DUNS #
City
Pine Bluff
State
AR
Country
United States
Zip Code
71601
Bakhtiar, R; Walker, R B; Dholakia, V N (1998) The effect of hydroxypropyl-beta-cyclodextrin on the kinetics of hydrolysis of an oxazolidine prodrug. Rapid Commun Mass Spectrom 12:1417-8
Walker, R B; Dholakia, V N; Brasfield, K L et al. (1998) Effect of hydroxypropyl-beta-cyclodextrin on the central stimulant activity of (-)-ephedrine and an oxazolidine prodrug in rats. Gen Pharmacol 30:725-31
Bakhtiar, R; Hop, C E; Walker, R B (1997) Effect of cyclodextrins on the hydrolysis of an oxazolidine prodrug of (1R,2S)-(-)-ephedrine-cis-2-(4-methoxyphenyl)-3, 4-dimethyl-5-phenyloxazolidine. Rapid Commun Mass Spectrom 11:598-602
Walker, R B; Fitz, L D; Williams, L M et al. (1996) The effect on ephedrine prodrugs on locomotor activity in rats. Gen Pharmacol 27:109-11
Walker, R B; Wood, D M; Akmal, M M et al. (1992) Effects of oxazolidines derived from (-) ephedrine in the rat. Gen Pharmacol 23:729-32
Walker, R B; Wood, D M; Akmal, M M (1990) Hyperthermic and anorectic effects of oxazolidines derived from L-ephedrine in rats. Life Sci 47:595-600