A variety of diseases of the cardio-vasculature including, spherical elliptocytosis, atherosclerosis, clotting disorders, and hypertension have been linked to structural alteration and dysfunction of the actin cytoskeleton and acto-myosin activity. Relevant to this application are the platelet mediated responses during clotting where these cells are structurally transformed, undergo degranulation, and extensively aggregate to participate in clot formation. Essential to these events is the restructuring of the actin cytoskeleton and its participation in exocytosis of intracellular granules. In the present application, we employ sea urchin coelomocytes as a model for platelet activation since, like platelets, they function in clot formation, undergo structural transformation reliant upon the actin cytoskeleton, and exhibit degranulation. However, unlike platelets, these cells are excellent models for high resolution light microscopic studies and correlative biochemical analyses. The planned training program will focus on experiments designed to define the associated of a 110 kDa unconventional myosin with intracellular vesicles and to determine unconventional myosin's role in vesicle motility and cytoskeletal reorganization. Within the scope of the project, the participants will be trained in a variety of methodologies including, but not limited to, protein fractionation, light and electron microscopy, fluorescence analog cytochemistry, immunocytochemistry, biophysics, and molecular biology. Results of ,D studies will expand our knowledge of how extracellular signals lead to cytoskeletal transformation, vesicle motility, and degranulation. Since, structural and regulatory pathways within the actin cytoskeleton appear to be highly conserved across diverse species, defining the mechanisms at work in coelomocyte activation will without doubt impact our understanding of equivalent events in mammalian platelets.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Rutgers University
United States
Zip Code
Baykal, Ahmet; Chakraborty, Sumit; Dodoo, Afua et al. (2006) Synthesis with good enantiomeric excess of both enantiomers of alpha-ketols and acetolactates by two thiamin diphosphate-dependent decarboxylases. Bioorg Chem 34:380-93
Mattson, Brandi J; Williams, Sharon E; Rosenblatt, Jay S et al. (2003) Preferences for cocaine- or pup-associated chambers differentiates otherwise behaviorally identical postpartum maternal rats. Psychopharmacology (Berl) 167:1-8
Gilchrist, Alan L; Annan Jr, Vidal (2002) Articulation effects in lightness: historical background and theoretical implications. Perception 31:141-50
Vathy, Ilona; Komisaruk, Barry R (2002) Differential effects of prenatal morphine exposure on analgesia produced by vaginocervical stimulation or systemic morphine administration in adult rats. Pharmacol Biochem Behav 72:165-70
Mattson, B J; Williams, S; Rosenblatt, J S et al. (2001) Comparison of two positive reinforcing stimuli: pups and cocaine throughout the postpartum period. Behav Neurosci 115:683-94
Duque, A; Balatoni, B; Detari, L et al. (2000) EEG correlation of the discharge properties of identified neurons in the basal forebrain. J Neurophysiol 84:1627-35
Komisaruk, B R; Rosenblatt, J S; Barona, M L et al. (2000) Combined c-fos and 14C-2-deoxyglucose method to differentiate site-specific excitation from disinhibition: analysis of maternal behavior in the rat. Brain Res 859:262-72
Caba, M; Komisaruk, B R; Beyer, C (1998) Analgesic synergism between AP5 (an NMDA receptor antagonist) and vaginocervical stimulation in the rat. Pharmacol Biochem Behav 61:45-8
Sansone, G R; Bianca, R; Cueva-Rolon, R et al. (1997) Cardiovascular responses to vaginocervical stimulation in the spinal cord-transected rat. Am J Physiol 273:R1361-6
Burroughs, L F; Fiber, J M; Swann, J M (1996) Neuropeptide Y in hamster limbic nuclei: lack of colocalization with substance P. Peptides 17:1053-62

Showing the most recent 10 out of 15 publications