Abstract

We have found a method to prepare polynucleotides so that they can be visualized in the Scanning Tunneling Microscope (STM). We want to study this system in an effort to examine its utility in an STM-based system for sequencing DNA. 2'-deoxynucleoside 5'-O-(1-thiotriphosphates) are substrates for the Thermus aquaticus DNA polymerase under the conditions of the polymerase chain reaction. Sulfur atoms in the resulting polynucleotides are located on the internucleotide phosphodiester groupings. This anionic sulfur binds divalent mercury in the form of 4- hydroxymercuribenzoate, a complex which is visible in STM. DNA sequence determinations have become one of the most important tools now available in biochemistry and molecular biology. The availability of such sequences is important in many different areas, including the study of infectious and hereditary disease as well as in the study of human ontogeny and molecular and organismic phylogeny.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
2S06GM008225-07
Application #
3856650
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Herbert H. Lehman College
Department
Type
DUNS #
620128301
City
New York
State
NY
Country
United States
Zip Code
10468

  Publications

Zheng, Zhi-Liang (2017) Ras and Rho GTPase regulation of Pol II transcription: A shortcut model revisited. Transcription 8:268-274
Zhang, Bo; Yang, Guohua; Chen, Yu et al. (2016) C-terminal domain (CTD) phosphatase links Rho GTPase signaling to Pol II CTD phosphorylation in Arabidopsis and yeast. Proc Natl Acad Sci U S A 113:E8197-E8206
Guerrero-Berroa, Elizabeth; Kluger, Alan; Schmeidler, James et al. (2014) Neuropsychological and neuropsychiatric prediction of global cognitive status among older Spanish-speaking Hispanics and English-speaking whites. J Geriatr Psychiatry Neurol 27:266-75
Zhang, Bo; Pasini, Rita; Dan, Hanbin et al. (2014) Aberrant gene expression in the Arabidopsis SULTR1;2 mutants suggests a possible regulatory role for this sulfate transporter in response to sulfur nutrient status. Plant J 77:185-97
Sailor, Kevin; Brooks, Patricia J (2014) Do part-whole relations produce facilitation in the picture-word interference task? Q J Exp Psychol (Hove) 67:1768-85
Gharbaran, Rajendra; Alvarado, Susana; Aisemberg, Gabriel O (2014) Regional and segmental differences in the embryonic expression of a putative leech Hox gene, Lox2, by central neurons immunoreactive to FMRFamide-like neuropeptides. Invert Neurosci 14:51-8
Gharbaran, Rajendra; Aisemberg, Gabriel O (2013) Identification of leech embryonic neurons that express a Hox gene required for the differentiation of a paired, segment-specific motor neuron. Int J Dev Neurosci 31:105-15
Gharbaran, Rajendra; Aisemberg, Gabriel O; Alvarado, Susana (2012) Segmental and regional differences in neuronal expression of the leech Hox genes Lox1 and Lox2 during embryogenesis. Cell Mol Neurobiol 32:1243-53
Yang, Hui; Figueroa, Mario; To, Satoshi et al. (2010) Benzophenones and biflavonoids from Garcinia livingstonei fruits. J Agric Food Chem 58:4749-55
Vallabhaneni, Ratnakar; Bradbury, Louis M T; Wurtzel, Eleanore T (2010) The carotenoid dioxygenase gene family in maize, sorghum, and rice. Arch Biochem Biophys 504:104-11

Showing the most recent 10 out of 67 publications