Abstract

The goal of this project is to investigate respective pathways involved in mRNA biogenesis, and identify potential sequences involved in mRNA trafficking in the nucleus. The results of the proposed studies will provide information on basic cellular functions. Heterokaryons being developed currently will be analyzed by in situ hybridization for the flow of newly created mRNAs' through the chick nucleus to identify other intranuclear RNA biogenesis pathway(s) (not just focus on the nucleolar pathway). In addition, using a chimeric B-galactosidase/mouse c-myc construct which has been shown, in HELA cells using in situ hybridization, to localize to the nucleolus, nested deletions of this chimeric vector will be used to identify c-myc sequence(s) causing intranuclear routing of that transcript. An undergraduate student is currently involved in several different aspects of the current grant, and will continue to be involved in this project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008248-11
Application #
6240360
Study Section
Project Start
1997-08-01
Project End
1998-07-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
11
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Morehouse School of Medicine
Department
Type
DUNS #
City
Atlanta
State
GA
Country
United States
Zip Code
30310

  Publications

Wilson, Nana O; Solomon, Wesley; Anderson, Leonard et al. (2013) Pharmacologic inhibition of CXCL10 in combination with anti-malarial therapy eliminates mortality associated with murine model of cerebral malaria. PLoS One 8:e60898
Igietseme, Joseph U; Omosun, Yusuf; Partin, James et al. (2013) Prevention of Chlamydia-induced infertility by inhibition of local caspase activity. J Infect Dis 207:1095-104
Wilson, Nana; Driss, Adel; Solomon, Wesley et al. (2013) CXCL10 gene promoter polymorphism -1447A>G correlates with plasma CXCL10 levels and is associated with male susceptibility to cerebral malaria. PLoS One 8:e81329
Kim, Teayoun; Zhelyabovska, Olga; Liu, Jian et al. (2013) Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR ?/? overexpression. Methods Mol Biol 952:57-65
Shelton, Martin N; Huang, Ming-Bo; Ali, Syed A et al. (2012) Secretion modification region-derived peptide disrupts HIV-1 Nef's interaction with mortalin and blocks virus and Nef exosome release. J Virol 86:406-19
Campbell, Patrick E; Isayev, Olexandr; Ali, Syed A et al. (2012) Validation of a novel secretion modification region (SMR) of HIV-1 Nef using cohort sequence analysis and molecular modeling. J Mol Model 18:4603-13
Liu, Mingli; Amodu, Audu S; Pitts, Sidney et al. (2012) Heme mediated STAT3 activation in severe malaria. PLoS One 7:e34280
Wilson, Nana O; Ceesay, Fatou K; Hibbert, Jacqueline M et al. (2012) Pregnancy outcomes among patients with sickle cell disease at Korle-Bu Teaching Hospital, Accra, Ghana: retrospective cohort study. Am J Trop Med Hyg 86:936-42
Johnson-Holiday, Crystal; Singh, Rajesh; Johnson, Erica et al. (2011) CCL25 mediates migration, invasion and matrix metalloproteinase expression by breast cancer cells in a CCR9-dependent fashion. Int J Oncol 38:1279-85
Jain, Vidhan; Lucchi, Naomi W; Wilson, Nana O et al. (2011) Plasma levels of angiopoietin-1 and -2 predict cerebral malaria outcome in Central India. Malar J 10:383

Showing the most recent 10 out of 122 publications