Abstract

The micromere-primary mesenchyme cell lineage of the sea urchin is a model system for investigating cellular and molecular aspects of cell differentiation. This lineage gives rise to the calcareous skeleton (spicule) of the embryo. Spicule formation requires intimate association between the primary mesenchyme and the extracellular matrix (E? of the embryo. We have developed a model system utilizing an exogenous ECM or serum which promotes mesenchyme differentiation in vitro.
Our first aim i s to isolate, characterize and raise antibodies to the spicule inducing activity (SIA) in the ECM.
Our second aim i s to identify transcripts in the micromeres which are induced by the serum or extracellular matrix. These transcripts presumably represent early gene products necessary for overt differentiation of this lineage.
The second aim i s independent of the first in that we need not identify the SIA in order to isolate the induced genes and their mRNAs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
1S06GM048135-01A1
Application #
3778455
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
California State University Hayward
Department
Type
DUNS #
194044335
City
Hayward
State
CA
Country
United States
Zip Code
94542

  Publications

Chu, Wally; Weerasekera, Akila; Kim, Chul-Hyun (2017) On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs. Biochem Biophys Res Commun 483:39-44
Porteus, Cosima; Hedrick, Michael S; Hicks, James W et al. (2011) Time domains of the hypoxic ventilatory response in ectothermic vertebrates. J Comp Physiol B 181:311-33
Barnes, Donna B; Murphy, Sheigla (2009) Reproductive decisions for women with HIV: motherhood's role in envisioning a future. Qual Health Res 19:481-91
Chen, Anna K; Hedrick, Michael S (2008) Role of glutamate and substance P in the amphibian respiratory network during development. Respir Physiol Neurobiol 162:24-31
Green, Lisa; Kim, Chul-Hyun; Bustamante, Carlos et al. (2008) Characterization of the mechanical unfolding of RNA pseudoknots. J Mol Biol 375:511-28
Hedrick, Michael S; Fahlman, Christian S; Bickler, Philip E (2005) Intracellular calcium and survival of tadpole forebrain cells in anoxia. J Exp Biol 208:681-6
Hedrick, Michael S (2005) Development of respiratory rhythm generation in ectothermic vertebrates. Respir Physiol Neurobiol 149:29-41
Winmill, Rachel E; Chen, Anna K; Hedrick, Michael S (2005) Development of the respiratory response to hypoxia in the isolated brainstem of the bullfrog Rana catesbeiana. J Exp Biol 208:213-22
Hedrick, Michael S; Chen, Anna K; Jessop, Kristy L (2005) Nitric oxide changes its role as a modulator of respiratory motor activity during development in the bullfrog (Rana catesbeiana). Comp Biochem Physiol A Mol Integr Physiol 142:231-40
Hedrick, Michael S; Winmill, Rachel E (2003) Excitatory and inhibitory effects of tricaine (MS-222) on fictive breathing in isolated bullfrog brain stem. Am J Physiol Regul Integr Comp Physiol 284:R405-12

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