Abstract

Eucaryotic genomes contain 30-100,000 genes, most of which have never been characterized. Current cDNA projects have the potential of cloning many of these genes within the next few years. The one time, single strand, partial sequence data generated by these projects will permit the identification of those genes with a high degree of potentially identifiable genes will be missed due to the limited and relatively inaccurate sequence data. This proposal is designed to determine how many potentially identifiable genes will be missed in current cDNA sequencing projects. Specifically, we will completely sequence 30 cDNAs from our pool of 100 novel, partially sequenced maize cDNAs. Expression analysis will also be conducted on each of these 30 cDNAs. These data will be extensively analyzed with the goal of functionally identifying these genes. The percentage of genes identified in these experiments should approximate the percentage identified when complete genomes are sequenced.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM048135-02
Application #
3756535
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
California State University Hayward
Department
Type
DUNS #
194044335
City
Hayward
State
CA
Country
United States
Zip Code
94542

  Publications

Chu, Wally; Weerasekera, Akila; Kim, Chul-Hyun (2017) On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs. Biochem Biophys Res Commun 483:39-44
Porteus, Cosima; Hedrick, Michael S; Hicks, James W et al. (2011) Time domains of the hypoxic ventilatory response in ectothermic vertebrates. J Comp Physiol B 181:311-33
Barnes, Donna B; Murphy, Sheigla (2009) Reproductive decisions for women with HIV: motherhood's role in envisioning a future. Qual Health Res 19:481-91
Chen, Anna K; Hedrick, Michael S (2008) Role of glutamate and substance P in the amphibian respiratory network during development. Respir Physiol Neurobiol 162:24-31
Green, Lisa; Kim, Chul-Hyun; Bustamante, Carlos et al. (2008) Characterization of the mechanical unfolding of RNA pseudoknots. J Mol Biol 375:511-28
Hedrick, Michael S (2005) Development of respiratory rhythm generation in ectothermic vertebrates. Respir Physiol Neurobiol 149:29-41
Winmill, Rachel E; Chen, Anna K; Hedrick, Michael S (2005) Development of the respiratory response to hypoxia in the isolated brainstem of the bullfrog Rana catesbeiana. J Exp Biol 208:213-22
Hedrick, Michael S; Chen, Anna K; Jessop, Kristy L (2005) Nitric oxide changes its role as a modulator of respiratory motor activity during development in the bullfrog (Rana catesbeiana). Comp Biochem Physiol A Mol Integr Physiol 142:231-40
Hedrick, Michael S; Fahlman, Christian S; Bickler, Philip E (2005) Intracellular calcium and survival of tadpole forebrain cells in anoxia. J Exp Biol 208:681-6
Hedrick, Michael S; Winmill, Rachel E (2003) Excitatory and inhibitory effects of tricaine (MS-222) on fictive breathing in isolated bullfrog brain stem. Am J Physiol Regul Integr Comp Physiol 284:R405-12

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