Quinoenzymes represent a widely distributed class of oxidoreductases with quinone cofactors that frequently occur in conjunction with metal ions as part of the catalytically functional redox active complex. Metal-quinone interactions have also been implicated in the mechanism of action of pharmacologic agents, including antitumor active antibiotics. The proposed project aims at structural characterization and mechanistic elucidation of synthetic quinone-metal complexes designed as bioorganic models to provide fundamental chemical precedents crucial for understanding the mechanism of action of biologically active metal-quinone complexes. Through the use of optical absorption spectroscopy, 1H- and 13C-NMR techniques as well as ESR methods the structural properties of the complexes will be delineated. Their redox chemistry will be developed using electrochemical methods as well as chemical reducing and oxidizing agents. The structural and chemical properties of the semiquinone radicals and their metal complexes will be elucidated using ESR spectroscopy in combination with electrochemical and kinetic studies. The methods developed in the project should provide useful precedents and may become applicable for studying metal-ligand interactions in other redox active heterocyclic systems of biological importance. The proposed project aims at providing research training to introduce minority students to biochemical research. The students will gain hands-on knowledge in modern techniques of organic synthesis, reaction kinetics, electrochemistry, ESR (including computer simulation of spectra), as well as routine analysis techniques such as FT-NMR, FT-IR and HPLC. Significantly, the students will be involved not only in the technical aspects of the proposed research but will also participate in the design of the experiments, interpretation of the results, will be expected to give scientific presentations and contribute to the writing of manuscripts for publication.

Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost
Name
California State University Northridge
Department
Type
DUNS #
055752331
City
Northridge
State
CA
Country
United States
Zip Code
91330
Alpizar, David; Laganá, Luciana; Plunkett, Scott W et al. (2018) Evaluating the eight-item Patient Health Questionnaire's psychometric properties with Mexican and Central American descent university students. Psychol Assess 30:719-728
Laganá, Luciana; Arellano, Kimberly; Alpizar, David (2017) Cognitive Functioning, Health Screening Behaviors and Desire to Improve One's Health in Diabetic versus Healthy Older Women. J Adv Med Med Res 23:
Mardirosian, Melina; Nalbandyan, Linette; Miller, Aaron D et al. (2016) Saw1 localizes to repair sites but is not required for recruitment of Rad10 to repair intermediates bearing short non-homologous 3' flaps during single-strand annealing in S. cerevisiae. Mol Cell Biochem 412:131-9
Giovannone, Dion; Ortega, Blanca; Reyes, Michelle et al. (2015) Chicken trunk neural crest migration visualized with HNK1. Acta Histochem 117:255-66
Benoun, Joseph M; Lalimar-Cortez, Danielle; Valencia, Analila et al. (2015) Rad7 E3 Ubiquitin Ligase Attenuates Polyubiquitylation of Rpn10 and Dsk2 Following DNA Damage in Saccharomyces cerevisiae. Adv Biol Chem 5:
Benes, Kylla M; Carpenter, Robert C (2015) Kelp canopy facilitates understory algal assemblage via competitive release during early stages of secondary succession. Ecology 96:241-51
Maciel, Michelle; Laganà, Luciana (2014) Older women's sexual desire problems: biopsychosocial factors impacting them and barriers to their clinical assessment. Biomed Res Int 2014:107217
Diamante, Graciel; Phan, Claire; Celis, Angie S et al. (2014) SAW1 is required for SDSA double-strand break repair in S. cerevisiae. Biochem Biophys Res Commun 445:602-7
Laganà, Luciana; Bloom, David William; Ainsworth, Andrew (2014) Urinary incontinence: its assessment and relationship to depression among community-dwelling multiethnic older women. ScientificWorldJournal 2014:708564
Laganá, Luciana; White, Theresa; Bruzzone, Daniel E et al. (2013) Exploring the Sexuality of African American Older Women. Br J Med Med Res 4:1129-1148

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