It is well-documented that obesity is associated with resistance to insulin- stimulated glucose uptake and may ultimately lead to the development of non-insulin-dependent diabetes. As over 80% of insulin-stimulated glucose clearance is mediated by skeletal muscle, it is of importance to evaluate interventions that may improve insulin action in skeletal muscle. Recently, leptin, the product of the ob gene, has been shown to reduce fat mass, hyperglycemia and hyperinsulinemia. Of particular interest, chronic leptin administration increases insulin-stimulated glucose uptake and transport in normal and insulin-resistant rat skeletal muscle. However, there has been little evidence presented to data indicating the mechanism by which chronic leptin administration improves insulin-mediated glucose clearance. Therefore, there are several novel questions that will be addressed in the proposed investigations. In the first investigation we address if chronic leptin administration alters components of the insulin- signaling cascade in normal and insulin-resistant skeletal muscle. In the second investigation we address if alterations in lipid metabolism, following chronic leptin treatment, can account for improvements in glucose metabolism in insulin-resistant skeletal muscle. In the third investigation we attempt to further our understanding how insulin- mediated glucose regulation is improved by interventions that appear to have similar effects on obesity and skeletal muscle insulin-resistance. The proposed investigations incorporate the use of experimental procedures that will serve to enhance and further develop the depth and quality of research performed in my laboratory. In addition, student trainees working on the project will be exposed to and acquire laboratory skills that will promote their intellectual maturity and prepare them for research careers at universities, research institutes and the biomedical industry.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
2S06GM048680-08
Application #
6369743
Study Section
Minority Programs Review Committee (MPRC)
Project Start
2001-07-01
Project End
2005-06-30
Budget Start
Budget End
Support Year
8
Fiscal Year
2001
Total Cost
Indirect Cost
Name
California State University Northridge
Department
Type
DUNS #
055752331
City
Northridge
State
CA
Country
United States
Zip Code
91330
Alpizar, David; Laganá, Luciana; Plunkett, Scott W et al. (2018) Evaluating the eight-item Patient Health Questionnaire's psychometric properties with Mexican and Central American descent university students. Psychol Assess 30:719-728
Laganá, Luciana; Arellano, Kimberly; Alpizar, David (2017) Cognitive Functioning, Health Screening Behaviors and Desire to Improve One's Health in Diabetic versus Healthy Older Women. J Adv Med Med Res 23:
Mardirosian, Melina; Nalbandyan, Linette; Miller, Aaron D et al. (2016) Saw1 localizes to repair sites but is not required for recruitment of Rad10 to repair intermediates bearing short non-homologous 3' flaps during single-strand annealing in S. cerevisiae. Mol Cell Biochem 412:131-9
Giovannone, Dion; Ortega, Blanca; Reyes, Michelle et al. (2015) Chicken trunk neural crest migration visualized with HNK1. Acta Histochem 117:255-66
Benoun, Joseph M; Lalimar-Cortez, Danielle; Valencia, Analila et al. (2015) Rad7 E3 Ubiquitin Ligase Attenuates Polyubiquitylation of Rpn10 and Dsk2 Following DNA Damage in Saccharomyces cerevisiae. Adv Biol Chem 5:
Benes, Kylla M; Carpenter, Robert C (2015) Kelp canopy facilitates understory algal assemblage via competitive release during early stages of secondary succession. Ecology 96:241-51
Maciel, Michelle; Laganà, Luciana (2014) Older women's sexual desire problems: biopsychosocial factors impacting them and barriers to their clinical assessment. Biomed Res Int 2014:107217
Diamante, Graciel; Phan, Claire; Celis, Angie S et al. (2014) SAW1 is required for SDSA double-strand break repair in S. cerevisiae. Biochem Biophys Res Commun 445:602-7
Laganà, Luciana; Bloom, David William; Ainsworth, Andrew (2014) Urinary incontinence: its assessment and relationship to depression among community-dwelling multiethnic older women. ScientificWorldJournal 2014:708564
Laganá, Luciana; White, Theresa; Bruzzone, Daniel E et al. (2013) Exploring the Sexuality of African American Older Women. Br J Med Med Res 4:1129-1148

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