Nucleotides such as ATP and GTP play a pivotal role in many biological activities. Nucleotide-cationinteractions are essential for the stabilization, function and regulation of nucleotides. The specific and oftenhighly selective interactions of nucleotides with the proteins are required to perform their biologicalfunctions. This proposal aims at characterizing the interactions of nucleotides with cations and proteins byNMR spectroscopy.
The specific aims of the proposed research are to investigate: (1) the interactions ofnucleotides with cations; (2) the mechanisms involved in the interactions of ATP/ADP with myosin andactin; and (3) the binding of ATP/GTP to bovine serum albumin (BSA) or ubiquitin. The studies involve NMRchemical shifts, T1, T2 relaxation and diffusion measurements. The NMR data will provide valuableinformation: a) to assess the stability of nucleotides; b) on the mechanisms of GTP/ATP hydrolysis; c) toevaluate the interplay of nucleotide-myosin/actin interactions in the ATP-driven cyclic associationdissociationof actin-myosin; and d) to verify whether BSA and ubiquitin are the candidates as ATPase orGTPase to induce ATP or GTP hydrolysis. These NMR studies have significance in biological andbiomedical sciences. The successful studies conducted by the PI for his MBRS-SCORE Program Pilot-Project (2001-2005) using NMR facility at Savannah State Univeristy formed the basis for the proposedresearch. If funded, this SCORE research project would enable the PI not only to continue his alreadyestablshied research involving NMR studies but also train students from the MARC U*STAR and NSFHBCU-UP Programs. The results from this study will be used for presenting at national and internationalconferences, for publication in peer-reviewed journals and for seeking extramural funding to continue theresearch beyond the MBRS-SCORE Program period.
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| Zhang, Xiaorong S; Choi, Jung H; Heinz, Josephine et al. (2006) Domain-specific positive selection contributes to the evolution of Arabidopsis leucine-rich repeat receptor-like kinase (LRR RLK) genes. J Mol Evol 63:612-21 |
