Modulation of the GABA-Ergic System by Pesticides in Rats of Different Ages

Uzodinma, John E.

Abstract

This project is designed to investigate the modulatory effects of pyrethroid and organophosphate compounds alone and in combination on alpha-aminobutyric acid (BABA) system in rat brain at different ages. It has been shown recently that these pesticides inhibit GABA-receptor complex and thus produce the convulsion and tremors since GABA-receptor complex and thus produce the convulsion and tremors since BABA functions as the inhibitory neurotransmitter. However, it is not clear at this time how these compounds affect the GABA system in the matching rat brain. Also, it is not known whether these two classes of compounds potentiate each others action in GABA system. Both in vitro and in vivo effects of these pesticides on GABA synthesizing (GAD) and catabolizing (GABA-T) enzymes, GABA receptor complex (3H-BABA binding, 3-H Flunitrazepine binding) and GABA release and uptake will be determine. The results thus obtained will help in the elucidation of the mechanism of action of these pesticides in matching rat brains. Another aim of the project is to provide minority students and faculty members at Jackson State University with opportunities for involvement in research activities in pesticide toxicity. Finally, statistical analysis will be carried out relative to the modulation of the GABA-ergic system by the interaction of pyrethroid and organophosphate compounds in rats at different ages.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Minority Biomedical Research Support - MBRS (S06)
• Project #: 5S06RR008047-18
• Application #: 3936742
• Support Year: 18
• Fiscal Year: 1988

Institution

• Name: Jackson State University
• DUNS #: 044507085
• City: Jackson
• State: MS
• Country: United States
• Zip Code: 39217

Publications

Desaiah, D; Pentyala, S N; Trottman, C H et al. (1991) Combined effects of carbon tetrachloride and chlordecone on calmodulin activity in gerbil brain. J Toxicol Environ Health 34:219-28

Jinna, R R; Uzodinma, J E; Desaiah, D (1989) Age-related changes in rat brain ATPases during treatment with chlordecone. J Toxicol Environ Health 27:199-208

Mohamed, A K; Pratt, J P; Nelson, F R (1987) Compatibility of Metarhizium anisopliae var. anisopliae with chemical pesticides. Mycopathologia 99:99-105

Moorthy, K S; Trottman, C H; Spann, C H et al. (1987) In vivo effects of toxaphene on calmodulin-regulated calcium-pump activity in rat brain. J Toxicol Environ Health 20:249-59

Nelson, F R; Holloway, D; Mohamed, A K (1986) A laboratory study of cyromazine on Aedes aegypti and Culex quinquefasciatus and its activity on selected predators of mosquito larvae. J Am Mosq Control Assoc 2:296-9

Dalhouse, A D; Langford, H G; Walsh, D et al. (1986) Angiotensin and salt appetite: physiological amounts of angiotensin given peripherally increase salt appetite in the rat. Behav Neurosci 100:597-602

Rao, K S; Trottman, C H; Morrow, W et al. (1986) Toxaphene inhibition of calmodulin-dependent calcium ATPase activity in rat brain synaptosomes. Fundam Appl Toxicol 6:648-53

Prasada Rao, K S; Chetty, C S; Trottman, C H et al. (1985) Effect of tricyclohexylhydroxytin on synaptosomal Ca2+-dependent ATP hydrolysis and rat brain subcellular calmodulin. Cell Biochem Funct 3:267-72

Brown, C E; Taylor, J M; Chan, L M (1985) The effect of pH on the interaction of substrates and effector to yeast and rabbit muscle pyruvate kinase. Biochim Biophys Acta 829:342-7

Nelson, F R; Mohamed, A K; Vattikutti, P (1985) Efficacy of three insect growth regulators on the development of Aedes aegypti. J Am Mosq Control Assoc 1:240-2