We propose to investigate the interfacial electron transfer reaction of several proteins in the absence and presence of redox mediators. The purpose is to gain an understanding of the energetics and kinetics of the direct and mediated electron transfer reaction of proteins at electrode surfaces. To accomplish this, a voltammetric study will be carried out to characterize the systems under study. Spectroelectrochemistry and rotating disk technique will be employed to determine thermodynamic and kinetic parameters, respectively. The proteins chosen for study were selected because of their ability to catalize the oxidation of substrates of physiological and/or pathological significance. These enzymatic reactions may represent the basis for the future development of voltammetric sensors that may find application in the clinical laboratory for the diagnosis of diseases and/or disorders caused by abnormal concentrations of metabolites.

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University of Puerto Rico Rio Piedras
San Juan
United States
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Rodriguez, A D; Ramirez, C; Shi, Y P (2000) The cumbiasins, structurally novel diterpenes possessing intricate carbocyclic skeletons from the West Indian sea whip Pseudopterogorgia elisabethae (Bayer). J Org Chem 65:6682-7
Rodriguez, A D; Ramirez, C (2000) A marine diterpene with a novel tetracyclic framework from the West Indian gorgonian octocoral Pseudopterogorgia elisabethae. Org Lett 2:507-10
Richardson, P L; Rodriguez, A D; Boulanger, A et al. (1998) Methoxyamericanolide B. Acta Crystallogr C 54 ( Pt 1):66-8
Figueroa, I D; el Baraka, M; Quinones, E et al. (1998) A fluorescent temperature probe based on the association between the excited states of 4-(N,N-dimethylamino)benzonitrile and beta-cyclodextrin. Anal Chem 70:3974-7
Rodriguez, A D; Soto, J J (1998) Pseudopterane and norcembrane diterpenoids from the Caribbean sea plume Pseudopterogorgia acerosa. J Nat Prod 61:401-4
Rodriguez, A D; Acosta, A L (1998) New cembranolides from the gorgonian Eunicea succinea. J Nat Prod 61:40-5
Rodriguez, A D; Cobar, O M; Padilla, O L et al. (1997) Calyxamines A and B, novel piperidine alkaloids from the Caribbean sea sponge Calyx podatypa. J Nat Prod 60:1331-3
Cobar, O M; Rodriguez, A D; Padilla, O L (1997) A new steroidal glycoside from a Caribbean gorgonian, Eunicea sp.1. J Nat Prod 60:1186-8
Rodriguez, A D; Gonzalez, E; Huang, S D (1997) Euniciniatin. Acta Crystallogr C 53 ( Pt 3):311-3
Rodriguez, A; Acosta, A L (1997) New cembranoid diterpenes and a geranylgeraniol derivative from the common Caribbean sea whip Eunicea succinea. J Nat Prod 60:1134-8

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