The Pacific Biosciences PacBio RS sequencing system will be used for high-throughput DNA sequencing in applications demanding long-range sequence information at the scale of tens of kilobases. The technology and characteristics of this sequencing technology are notably different than for other sequencing methodologies. In particular, the ability to obtain long sequence reads affords several critical advantages for a number of applications, including (1) the sequencing of novel genomes, (2) EST and transcriptome characterization in organisms lacking a sequenced or well-mapped genome, (3) the analysis of alternative pre-mRNA splicing, (4) amplicon sequencing. In addition to longer read lengths, the PacBio sequencing technology also provides rapid turnaround time. The PacBio RS will be applied to a diverse array of NIH-funded projects as well as other projects of high relevance to biomedical science, including the characterization of multigene families involved in sensory perception;the elucidation of the patterns and mechanisms of alternative pre-mRNA splicing;the characterization of genomes of genetic and evolutionary models of human development and disease;an analysis of the genetic loci associated with cancer;and an investigation of the susceptibility of mammalian cells to nutritional ions and environmental toxins. There are currently no PacBio sequencers on the UC Berkeley campus. The proposed instrument would be located close to its users as part of the Coates Genome Sequencing Center, and managed as part of the unified campus-wide sequencing core facility and associated Computational Genomics Resource Laboratory. Acquisition of this instrument will support researchers pursuing these projects, which promise to illuminate the fundamental mechanisms underlying human biology and disease.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Birken, Steven
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Berkeley
Schools of Arts and Sciences
United States
Zip Code
Elya, Carolyn; Lok, Tin Ching; Spencer, Quinn E et al. (2018) Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory. Elife 7:
Akagi, Takashi; Henry, Isabelle M; Ohtani, Haruka et al. (2018) A Y-Encoded Suppressor of Feminization Arose via Lineage-Specific Duplication of a Cytokinin Response Regulator in Kiwifruit. Plant Cell 30:780-795
Coulson, Rochelle L; Yasui, Dag H; Dunaway, Keith W et al. (2018) Snord116-dependent diurnal rhythm of DNA methylation in mouse cortex. Nat Commun 9:1616
Bryson Jr, Robert W; Wood, Dustin A; Graham, Matthew R et al. (2018) Genome-wide SNP data and morphology support the distinction of two new species of Kovarikia Soleglad, Fet & Graham, 2014 endemic to California (Scorpiones, Vaejovidae). Zookeys :79-106
Vogel Ciernia, Annie; Careaga, Milo; LaSalle, Janine M et al. (2018) Microglia from offspring of dams with allergic asthma exhibit epigenomic alterations in genes dysregulated in autism. Glia 66:505-521
Carrico, Adam W; Flentje, Annesa; Kober, Kord et al. (2018) Recent stimulant use and leukocyte gene expression in methamphetamine users with treated HIV infection. Brain Behav Immun 71:108-115
Wakasa, Yuhya; Kawakatsu, Taiji; Harada, Takeo et al. (2018) Transgene-independent heredity of RdDM-mediated transcriptional gene silencing of endogenous genes in rice. Plant Biotechnol J 16:2007-2015
Frost, Jennifer M; Kim, M Yvonne; Park, Guen Tae et al. (2018) FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis. Proc Natl Acad Sci U S A 115:E4720-E4729
Fraser, Devaughn; Mouton, Alice; Serieys, Laurel E K et al. (2018) Genome-wide expression reveals multiple systemic effects associated with detection of anticoagulant poisons in bobcats (Lynx rufus). Mol Ecol 27:1170-1187
Price, April E; Shamardani, Kiarash; Lugo, Kyler A et al. (2018) A Map of Toll-like Receptor Expression in the Intestinal Epithelium Reveals Distinct Spatial, Cell Type-Specific, and Temporal Patterns. Immunity 49:560-575.e6

Showing the most recent 10 out of 43 publications