The University of California at Davis (UCD) is a diverse educational institution recognized for excellence in medical, veterinary and agricultural research. In 1998, the UCD Flow Cytometry Shared Resource Laboratory received S10 program support (S10-RR012964-01) to purchase a multi-laser, 10-color Cytomation MoFlo high- speed cell sorter. Specific features in the design of the MoFlo cell sorter are ideally suite for use in a shared resource lab, facilitating the analysis and sorting of a variety of cells from human and animal tissues, as well as microparticles, microorganisms, and plant cell protoplasts. For 15 years, the MoFlo has supported the research of UCD investigators across a wide range of multidisciplinary programs in Davis and at the UCD Medical Center in Sacramento, including the Schools of Medicine and Veterinary Medicine, NCI Comprehensive Cancer Center and the California National Primate Research Center. However, after years of reliable service, the original legacy MoFlo is now in need of replacement. Key component systems are aged and obsolete, have become unreliable and are expensive or impossible to maintain, particularly the water-cooled gas lasers. Further, the large size of the legacy MoFlo does not allow for enclosure in a biosafety containment cabinet and fails to meet the modern biosafety recommendations of the International Society for the Advancement of Cytometry and the newly approved NIH Biosafety Policy for Cell Sorters. The proposed replacement instrumentation, the Beckman Coulter AstriosEQ, offers modern components but uses the same stable, reliable fluidic design that is the hallmark of MoFlo cell sorters. The AstriosEQ will be equipped with 4 lasers and 16 photodetectors to detect commonly used fluorescent probes and proteins and will be housed in a biosafety containment hood with aerosol management to ensure safe sorting of biohazardous samples. The success of NIH supported biological research at UC Davis depends on the uninterrupted availability of a flexible, reliable, and safe cell sorter. The AstriosEQ willbe housed in, managed and maintained by the Davis campus Shared Flow Cytometry Laboratory so that UCD investigators have access to cell sorting instrumentation and flow cytometry expertise for years to come.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Special Emphasis Panel (ZRG1-CB-D (31))
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Levy, Abraham
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University of California Davis
Schools of Medicine
United States
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Seo, Jai Woong; Tavaré, Richard; Mahakian, Lisa M et al. (2018) CD8+ T-Cell Density Imaging with 64Cu-Labeled Cys-Diabody Informs Immunotherapy Protocols. Clin Cancer Res 24:4976-4987
Dou, John; Schmidt, Rebecca J; Benke, Kelly S et al. (2018) Cord blood buffy coat DNA methylation is comparable to whole cord blood methylation. Epigenetics 13:108-116
McGee, Heather M; Daly, Megan E; Azghadi, Sohelia et al. (2018) Stereotactic Ablative Radiation Therapy Induces Systemic Differences in Peripheral Blood Immunophenotype Dependent on Irradiated Site. Int J Radiat Oncol Biol Phys 101:1259-1270
Wang, Yuru; Park, SeHee; Beal, Peter A (2018) Selective Recognition of RNA Substrates by ADAR Deaminase Domains. Biochemistry 57:1640-1651
Kiniry, Brenna E; Li, Shengbin; Ganesh, Anupama et al. (2018) Detection of HIV-1-specific gastrointestinal tissue resident CD8+ T-cells in chronic infection. Mucosal Immunol 11:909-920
Besprozvannaya, Marina; Dickson, Eamonn; Li, Hao et al. (2018) GRAM domain proteins specialize functionally distinct ER-PM contact sites in human cells. Elife 7:
Fletcher, Kyle; Klosterman, Steven J; Derevnina, Lida et al. (2018) Comparative genomics of downy mildews reveals potential adaptations to biotrophy. BMC Genomics 19:851
Gillies, Taryn E; Pargett, Michael; Minguet, Marta et al. (2017) Linear Integration of ERK Activity Predominates over Persistence Detection in Fra-1 Regulation. Cell Syst 5:549-563.e5
Kiniry, B E; Ganesh, A; Critchfield, J W et al. (2017) Predominance of weakly cytotoxic, T-betLowEomesNeg CD8+ T-cells in human gastrointestinal mucosa: implications for HIV infection. Mucosal Immunol 10:1008-1020
Wang, Yuru; Beal, Peter A (2016) Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method. Nucleic Acids Res 44:9872-9880

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