This goal of this application is to acquire a state-of-the-art laser-scanning confocal microscope to support the research of 11 NIH-funded investigators at the Tulane National Primate Research Center (TNPRC). The TNPRC is an NIH-supported national resource with a mission that integrates research, training and service in biomedical research to improve human and animal health through basic and applied biomedical research. The existing multiuser confocal system, a Leica TCS SP2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. While the Leica TCS SP2 was state-of-the-art when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity, imaging quality, speed, and versatility. Furthermore, Leica will cease to provide service for the SP2 within the next year. In this application we present research projects from 11 investigators (8 Major users and 3 Minor users) whose work is performed at the TNPRC. These projects are primarily focused on infectious diseases and immunology which are areas of exceptional expertise at the TNPRC and that attract national and international collaborators. Each of the users has a strong history of NIH funding and needs for advanced imaging technology. We propose purchasing a Leica TCS SP 8 five-channel confocal microscopy imaging system with an inverted microscope, 6 strong laser lines (diode: 405 nm, Blue Ar: 458, 488, 514 nm, yellow diode: 561 nm, and red HeNe: 633 nm) with AOBS, and two AOTF (405 nm and visible), 3 hybrid GaAsP spectral detectors and 2 HS PMT and easy to use advanced software all of which are essential to the projects of the users. Compared to other premiere systems on the market we feel the Leica TCS SP 8 offers the best overall system in terms of quality, options, service, and price. It also is highly flexible and will be able to provide new services as required by the users. This new confocal microscope system is essential not only to the major and minor users listed in the application but to the mission of the TNPRC as a national resource for biomedical research using nonhuman primates. The availability of a newer, more reliable, sophisticated and versatile instrument such as the SP 8 is likely to attract additional investigators that require ths technology leading to new collaborations further expanding the scope of nonhuman primate research at the TNPRC.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Levy, Abraham
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Tulane University
Schools of Medicine
New Orleans
United States
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Crossland, Nicholas A; Alvarez, Xavier; Embers, Monica E (2018) Late Disseminated Lyme Disease: Associated Pathology and Spirochete Persistence Posttreatment in Rhesus Macaques. Am J Pathol 188:672-682
Xu, Huanbin; Ziani, Widade; Shao, Jiasheng et al. (2018) Impaired Development and Expansion of Germinal Center Follicular Th Cells in Simian Immunodeficiency Virus-Infected Neonatal Macaques. J Immunol 201:1994-2003
Kumar, Vinay; Mansfield, Joshua; Fan, Rong et al. (2018) miR-130a and miR-212 Disrupt the Intestinal Epithelial Barrier through Modulation of PPAR? and Occludin Expression in Chronic Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol 200:2677-2689
Kuroda, Marcelo J; Sugimoto, Chie; Cai, Yanhui et al. (2018) High Turnover of Tissue Macrophages Contributes to Tuberculosis Reactivation in Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Infect Dis 217:1865-1874
McGary, C S; Alvarez, X; Harrington, S et al. (2017) The loss of CCR6+ and CD161+ CD4+ T-cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal Immunol 10:1082-1096
Sugimoto, Chie; Merino, Kristen M; Hasegawa, Atsuhiko et al. (2017) Critical Role for Monocytes/Macrophages in Rapid Progression to AIDS in Pediatric Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Virol 91:
Hudock, Teresa A; Foreman, Taylor W; Bandyopadhyay, Nirmalya et al. (2017) Hypoxia Sensing and Persistence Genes Are Expressed during the Intragranulomatous Survival of Mycobacterium tuberculosis. Am J Respir Cell Mol Biol 56:637-647
Xu, Huanbin; Wang, Xiaolei; Malam, Naomi et al. (2016) Persistent Simian Immunodeficiency Virus Infection Drives Differentiation, Aberrant Accumulation, and Latent Infection of Germinal Center Follicular T Helper Cells. J Virol 90:1578-87
Foreman, Taylor W; Mehra, Smriti; LoBato, Denae N et al. (2016) CD4+ T-cell-independent mechanisms suppress reactivation of latent tuberculosis in a macaque model of HIV coinfection. Proc Natl Acad Sci U S A 113:E5636-44
Liu, L; Wei, Q; Nishiura, K et al. (2016) Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques. Mucosal Immunol 9:1089-101

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