We request funds for the purchase of a Leica EM UC7 ultramicrotome for sectioning biological and biomaterials samples at room temperatures. The new instrument will help provide for the ultramicrotomy needs of over 60 user laboratories at the University of Massachusetts Medical School (UMMS) and the Graduate School of Biomedical Sciences (GSBS). The new ultramicrotome will be housed in the Core Electron Microscopy Facility of the Medical School, and will replace a 30-year old Reichert-Jung Ultracut E, that while still functioning, is now considered obsolete, has become unreliable, and is no longer supported by the manufacturer (parts or service). Ultramicrotomes are used to cut thin sections of cells and tissues that are required for understanding their detailed structure and the changes in structure that underlie human disease. Many tissue-engineering strategies involve the delivery of cells via porous polymer scaffolds. Obtaining sections of the emerging tissue is necessary to analyze the characteristics of the microscopic environment. The Core Electron Microscopy facility supports projects ranging from studies of the structure of muscle, microtubule formation and stabilization, stem cell and germ cell differentiation, cilia and flagell structure, just to mention a few. All are important topics in the understanding of the normal function and structure of the human body and all require ultramicrotomy. The Leica EM UC7 Ultra-microtome offers a range of outstanding features and benefits over our old Reichert-Jung Ultracut E model. Each user, from absolute beginner students to our most skilled ultra- microtomist will benefit from this modern instrument. The eucentric movement of the viewing system allows examination of sections even with lowered water level (as with Lowycryl, and LR White resins, used in immune-labeling projects). The touch sensitive control unit of the Leica EM UC7 enables fast and safe alignment of knife and specimen with help files and prompts, simplifying the trimming- process while preparing samples for sectioning. Funding this grant will help us provide state-of-the-art instrumentation for our shared research Electron Microscopy Core Facility, utilized by over 100 individual researchers and their graduate students as well as the faculty and graduate students at several colleges in the surrounding area. The new instrument will greatly enhance the ability of our investigators and students to carry out their microtomy-related studies, it will greatly improve the quality and speed of data collection, and it will make possible types of observations that are impossible with our older (obsolete) instrument.

Public Health Relevance

We are requesting funds for the purchase of a new Ultramicrotome that will support the research needs of the many NIH-funded investigators at UMass Medical School (UMMS) and Graduate School of Biomedical sciences (GSBS). Ultramicrotomes are used to cut thin sections of cells and tissues that are required for understanding their detailed structure and the changes in structure that underlie disease. Projects outlined here, include studies of muscle, cilia and flagella, autophagy and programmed cell death, fusion proteins in the ovarian germ cells, and the characterization of microtubule-associated proteins that stabilize microtubules in infected cells, all essential to the normal functioning of the human body.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-CB-S (30))
Program Officer
Levy, Abraham
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Massachusetts Medical School Worcester
Anatomy/Cell Biology
Schools of Medicine
United States
Zip Code
Shin, Young C; Bischof, Georg F; Lauer, William A et al. (2018) A recombinant herpesviral vector containing a near-full-length SIVmac239 genome produces SIV particles and elicits immune responses to all nine SIV gene products. PLoS Pathog 14:e1007143
Brown, Jason M; Mosley, Matthew; Montes-Berrueta, Daniela et al. (2017) Characterization of a new oda3 allele, oda3-6, defective in assembly of the outer dynein arm-docking complex in Chlamydomonas reinhardtii. PLoS One 12:e0173842
Zhang, Zhengang; Li, Wei; Zhang, Yong et al. (2016) Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice. Mol Biol Cell :
Rao, Kollu N; Zhang, Wei; Li, Linjing et al. (2016) Ciliopathy-associated protein CEP290 modifies the severity of retinal degeneration due to loss of RPGR. Hum Mol Genet 25:2005-2012
San Agustin, Jovenal T; Klena, Nikolai; Granath, Kristi et al. (2016) Genetic link between renal birth defects and congenital heart disease. Nat Commun 7:11103
Kramer, Nicholas J; Carlomagno, Yari; Zhang, Yong-Jie et al. (2016) Spt4 selectively regulates the expression of C9orf72 sense and antisense mutant transcripts. Science 353:708-12
Li, Yihang; Hassinger, Linda; Thomson, Travis et al. (2016) Lamin Mutations Accelerate Aging via Defective Export of Mitochondrial mRNAs through Nuclear Envelope Budding. Curr Biol 26:2052-2059
Petit, Lolita; Khanna, Hemant; Punzo, Claudio (2016) Advances in Gene Therapy for Diseases of the Eye. Hum Gene Ther 27:563-79
Kubo, Tomohiro; Brown, Jason M; Bellve, Karl et al. (2016) Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. J Cell Sci 129:2106-19
Budnik, Vivian; Ruiz-CaƱada, Catalina; Wendler, Franz (2016) Extracellular vesicles round off communication in the nervous system. Nat Rev Neurosci 17:160-72

Showing the most recent 10 out of 13 publications