We are requesting funds to acquire a high-sensitivity mass spectrometer Qtrap 6500+ from SCIEX. The instrument will support users involved in anti-infective research, with a focus on the quantitation and spatial distribution of antibiotics as well as investigational new drugs. The instrument will be located in the Analytical and Imaging Facility of the Public Health Research Institute at the New Jersey Medical School of Rutgers University. A team of qualified personnel is available on-site to manage and operate the instrument as part of a well-established analytical platform supported by the NJMS and PHRI. The research projects include the quantitation of drugs active against HIV, tuberculosis and fungal pathogens in plasma and at the sites of infection, in both clinical samples and animal models. The highly sensitive instrument will overcome many of the current limitations we experience due to either small sample volumes or low drug levels. Working with small sample volumes increases throughput, reduces the number of animals required in pharmacokinetic and pharmacodynamic studies, and enables drug quantitation in challenging but important matrices such as vaginal swabs and dried blood spots. We have recently developed a novel method to achieve spatial quantitation of drugs and lipids using laser-capture microdissection combined with high- pressure liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The Qtrap 6500+ will leverage the capabilities of this pioneering method since it will allow for reducing the amount of micro-dissected material required to achieve accurate quantitation, thus improving spatial resolution. Another set of projects focus on the detection and quantitation of biomarkers of pathology, disease progression and drug response in plasma, urine and tissues. Again, the high sensitivity of the proposed instrument will be key to the success of these projects due to the low abundance of some of these biomarkers, and the small volumes of samples collected by laser-capture microdissection. A high accuracy mass spectrometer in our well-supported facility will expand and promote research opportunities for both neglected and less neglected infectious diseases. With the new Sciex 6500, our analytic capabilities will reach the next level of sensitivity and spatial quantitation and place PHRI on the global map of anti-infective research.

Public Health Relevance

Our group at the Public Health Research Institute of Rutgers University is requesting a highly sensitive mass spectrometer to measure the concentration of drugs and drug candidates in biological samples. The instrument will be shared by 13 users all involved in infectious disease research, and will facilitate the discovery of better antibiotics against tuberculosis and other high-threat pathogens.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD023524-01
Application #
9274490
Study Section
Special Emphasis Panel (ZRG1-BCMB-T (31)I)
Program Officer
Horska, Alena
Project Start
2017-04-01
Project End
2018-03-31
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
1
Fiscal Year
2017
Total Cost
$526,083
Indirect Cost
Name
Rutgers University
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
078795851
City
Newark
State
NJ
Country
United States
Zip Code
07103
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