This application is for the acquisition of a continuous flow gas chromatograph combustion/ paralysis-isotope ratio mass spectrometer (GC-CF-IRMS) with modules for the on-line assay of low enrichments of compounds labeled with 13C, 15N, 18O, and 2H. The applicants are five established and two junior faculties of Case Western Reserve University and two colleagues from Youngstown State University (Ohio) and the University of Oklahoma, all with expertise in gas chromatography-mass spectrometry. The instrument will be used for basic science and clinical investigations on enzymatic reaction mechanisms, gluconeogenesis, lipogenesis, ketone body metabolism, protein metabolism, fatty acid oxidation, and inborn metabolic disorders. No such instrument is presently available at CWRU or in Greater Cleveland. The instrument will be set up in the Center for Metabolic Mass Spectrometry of the Department of Nutrition of CWRU. It will be made available to all scientists of the Cleveland area. The costs of operating and maintaining the instrument will be shared by the users. The availability of this instrument will considerably increase the scope of the basic science and clinical investigations conducted by the applicants.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR017865-01
Application #
6580510
Study Section
Special Emphasis Panel (ZRG1-BECM (02))
Program Officer
Tingle, Marjorie
Project Start
2003-05-01
Project End
2004-04-30
Budget Start
2003-05-01
Budget End
2004-04-30
Support Year
1
Fiscal Year
2003
Total Cost
$250,000
Indirect Cost
Name
Case Western Reserve University
Department
Nutrition
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106