Abstract

This is an application for funds to purchase an ABI PRISM 7900 Sequence Detection System for use by 7 basic science researchers funded by 9 NIH R01 grants. Detection of DNA or RNA by real time PCR using this instrument has several advantages over conventional semiquantitative PCR in providing increased sensitivity and specificity as well as reducing the time of the assay and increasing safety by eliminating the requirement for radioactive probing. The projects described by the investigators would therefore be able to address issues of quantitation of gene expression with a much higher degree of accuracy and facility, improving safety and increasing the information to be gained from each study. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR017895-01
Application #
6579972
Study Section
Special Emphasis Panel (ZRG1-SSS-G (30))
Program Officer
Tingle, Marjorie
Project Start
2003-04-01
Project End
2004-03-31
Budget Start
2003-04-01
Budget End
2004-03-31
Support Year
1
Fiscal Year
2003
Total Cost
$135,937
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Pediatrics
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Mayle, Allison; Luo, Min; Jeong, Mira et al. (2013) Flow cytometry analysis of murine hematopoietic stem cells. Cytometry A 83:27-37
Lin, Kuanyin K; Goodell, Magaret A (2011) Detection of hematopoietic stem cells by flow cytometry. Methods Cell Biol 103:21-30
Rossi, Lara; Challen, Grant A; Sirin, Olga et al. (2011) Hematopoietic stem cell characterization and isolation. Methods Mol Biol 750:47-59
Ergen, Aysegul V; Goodell, Margaret A (2010) Mechanisms of hematopoietic stem cell aging. Exp Gerontol 45:286-90