Funding is requested for a dedicated 500 MHz high-resolution, solution and magic angle spinning (MAS) Nuclear Magnetic Resonance (NMR) spectrometer. The proposed instrument will be a crucial component in facilitating the development and synthesis of novel molecular imaging contrast agents and for the identification of biomarkers for specific disease states and biological processes. The NMR spectrometer will serve a large NIH-funded geographically diverse user community encompassing a wide variety of biomedical disciplines. We currently serve users both within the MGH/Harvard/MIT system as well as from around the greater Boston/Cambridge region. However, current users do not have sufficient magnet access, and many others would like to take similar advantage of our facilities, but cannot readily do so. Our current wide-bore 600 MHz MR instrument, which is used for liquids, HR-MAS, and CP-MAS NMR spectroscopy as well as MRI, is overscheduled, operating on a 24x7 basis with reservations needing to be made up to 3 months in advance. The long scan times required for obtaining high resolution MR images and the high demand for scan time make it impossible to use the instrument for regular and routine NMR chemical analysis of contrast agents and biological tissues. The requested equipment would greatly expand our capacity to serve the local NIH- funded user community, as well as offer important analytical chemistry capability to the rapidly expanding user base with molecular imaging interests. In particular, the proposed instrument would allow for """"""""walk-on"""""""" scan time for the rapid characterization of intermediates in reaction pathways of novel molecular imaging probes currently being developed and for the identification of biomarkers for different disease states. The institutions to be served include MGH (radiology, neurology, pathology, and surgery), Children's Hospital (orthopaedic surgery), Boston University and Northeastern University. Projects requiring solution NMR spectroscopy include the development and synthesis of (1) dual MRI-histology contrast agents for brain molecular imaging, (2) novel blood-brain barrier permeable contrast agents for brain molecular imaging, (3) amyloid plaque targeting contrast agents for Alzheimer's disease studies, (4) targeted drug delivery vehicles, (5) lymph node specific contrast agents, and the identification of biomarkers associated with (6) stroke and (7) Alzheimer's disease. Projects requiring HR-MAS NMR spectroscopy include identifying biomarkers for (1) breast cancer, (2) inflammatory response in burn tissue (3) HIV injured brain tissue, and (4) ostereoporosis. ? ? ?

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-SBIB-N (30))
Program Officer
Tingle, Marjorie
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Massachusetts General Hospital
United States
Zip Code
Farrar, Christian T; Gale, Eric M; Kennan, Richard et al. (2018) CM-101: Type I Collagen-targeted MR Imaging Probe for Detection of Liver Fibrosis. Radiology 287:581-589
Gale, Eric M; Wey, Hsiao-Ying; Ramsay, Ian et al. (2018) A Manganese-based Alternative to Gadolinium: Contrast-enhanced MR Angiography, Excretion, Pharmacokinetics, and Metabolism. Radiology 286:865-872
Dai, Lixiong; Jones, Chloe M; Chan, Wesley Ting Kwok et al. (2018) Chiral DOTA chelators as an improved platform for biomedical imaging and therapy applications. Nat Commun 9:857
Adams, Casey J; Wilson, Justin J; Boros, Eszter (2017) Multifunctional Desferrichrome Analogues as Versatile 89Zr(IV) Chelators for ImmunoPET Probe Development. Mol Pharm 14:2831-2842
Boros, Eszter; Srinivas, Raja; Kim, Hee-Kyung et al. (2017) Intramolecular Hydrogen Bonding Restricts Gd-Aqua-Ligand Dynamics. Angew Chem Int Ed Engl 56:5603-5606
Van de Bittner, Genevieve C; Riley, Misha M; Cao, Luxiang et al. (2017) Nasal neuron PET imaging quantifies neuron generation and degeneration. J Clin Invest 127:681-694
Hooker, Jacob M; Strebl, Martin G; Schroeder, Frederick A et al. (2017) Imaging cardiac SCN5A using the novel F-18 radiotracer radiocaine. Sci Rep 7:42136
Polasek, Miloslav; Yang, Yan; Schühle, Daniel T et al. (2017) Molecular MR imaging of fibrosis in a mouse model of pancreatic cancer. Sci Rep 7:8114
Oliveira, Bruno L; Caravan, Peter (2017) Peptide-based fibrin-targeting probes for thrombus imaging. Dalton Trans 46:14488-14508
Waghorn, Philip A; Jones, Chloe M; Rotile, Nicholas J et al. (2017) Molecular Magnetic Resonance Imaging of Lung Fibrogenesis with an Oxyamine-Based Probe. Angew Chem Int Ed Engl 56:9825-9828

Showing the most recent 10 out of 81 publications