This is an application requesting funds to purchase instrumentation to conduct high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). The instrument will be located in an existing biological mass spectrometry facility on the Piscataway campus of Robert Wood Johnson Medical School (RWJMS) and Rutgers University. A high resolution LC-MS/MS instrument will greatly expand the capabilities of the facility to meet the increasing research needs of 31 NIH-funded investigators. Funds from NIH along with institutions support will allow the acquisition of a ThermoFisher LTQ Orbitrap XL ETD mass spectrometer, a Proxeon nanospray source, and a Dionex Ultimate 3000 HPLC system. There are no comparable instruments at RWJMS and Rutgers. The instrument will be used for characterization of post-translational modifications on proteins, quantitative analysis/comparative proteomics, crosslinking analysis, solvent accessibility studies, and other application that require high-resolution LC-MS/MS capabilities. This will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.

Public Health Relevance

This is an application requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by 31 NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-S (30))
Program Officer
Birken, Steven
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Medicine & Dentistry of NJ
Schools of Medicine
United States
Zip Code
Solé-Domènech, Santiago; Rojas, Ana V; Maisuradze, Gia G et al. (2018) Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar A? by multiple endoproteolytic cleavages within the ?-sheet domain. Proc Natl Acad Sci U S A 115:1493-1498
Sleat, David E; Tannous, Abla; Sohar, Istvan et al. (2017) Proteomic Analysis of Brain and Cerebrospinal Fluid from the Three Major Forms of Neuronal Ceroid Lipofuscinosis Reveals Potential Biomarkers. J Proteome Res 16:3787-3804
Jadot, Michel; Boonen, Marielle; Thirion, Jaqueline et al. (2017) Accounting for Protein Subcellular Localization: A Compartmental Map of the Rat Liver Proteome. Mol Cell Proteomics 16:194-212
Meng, Yu; Wiseman, Jennifer A; Nemtsova, Yuliya et al. (2017) A Basic ApoE-Based Peptide Mediator to Deliver Proteins across the Blood-Brain Barrier: Long-Term Efficacy, Toxicity, and Mechanism. Mol Ther 25:1531-1543
Tan, William; Schauder, Curtis; Naryshkina, Tatyana et al. (2016) Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function. Dev Biol 410:202-212
Sleat, David E; Wiseman, Jennifer A; Sohar, Istvan et al. (2012) Proteomic analysis of mouse models of Niemann-Pick C disease reveals alterations in the steady-state levels of lysosomal proteins within the brain. Proteomics 12:3499-509
Calkins, Marcus J; Reddy, P Hemachandra (2011) Amyloid beta impairs mitochondrial anterograde transport and degenerates synapses in Alzheimer's disease neurons. Biochim Biophys Acta 1812:507-13