This is an application requesting funds to purchase instrumentation to conduct high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). The instrument will be located in an existing biological mass spectrometry facility on the Piscataway campus of Robert Wood Johnson Medical School (RWJMS) and Rutgers University. A high resolution LC-MS/MS instrument will greatly expand the capabilities of the facility to meet the increasing research needs of 31 NIH-funded investigators. Funds from NIH along with institutions support will allow the acquisition of a ThermoFisher LTQ Orbitrap XL ETD mass spectrometer, a Proxeon nanospray source, and a Dionex Ultimate 3000 HPLC system. There are no comparable instruments at RWJMS and Rutgers. The instrument will be used for characterization of post-translational modifications on proteins, quantitative analysis/comparative proteomics, crosslinking analysis, solvent accessibility studies, and other application that require high-resolution LC-MS/MS capabilities. This will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.
This is an application requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by 31 NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.
|Solé-Domènech, Santiago; Rojas, Ana V; Maisuradze, Gia G et al. (2018) Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar A? by multiple endoproteolytic cleavages within the ?-sheet domain. Proc Natl Acad Sci U S A 115:1493-1498|
|Sleat, David E; Tannous, Abla; Sohar, Istvan et al. (2017) Proteomic Analysis of Brain and Cerebrospinal Fluid from the Three Major Forms of Neuronal Ceroid Lipofuscinosis Reveals Potential Biomarkers. J Proteome Res 16:3787-3804|
|Jadot, Michel; Boonen, Marielle; Thirion, Jaqueline et al. (2017) Accounting for Protein Subcellular Localization: A Compartmental Map of the Rat Liver Proteome. Mol Cell Proteomics 16:194-212|
|Meng, Yu; Wiseman, Jennifer A; Nemtsova, Yuliya et al. (2017) A Basic ApoE-Based Peptide Mediator to Deliver Proteins across the Blood-Brain Barrier: Long-Term Efficacy, Toxicity, and Mechanism. Mol Ther 25:1531-1543|
|Tan, William; Schauder, Curtis; Naryshkina, Tatyana et al. (2016) Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function. Dev Biol 410:202-212|
|Sleat, David E; Wiseman, Jennifer A; Sohar, Istvan et al. (2012) Proteomic analysis of mouse models of Niemann-Pick C disease reveals alterations in the steady-state levels of lysosomal proteins within the brain. Proteomics 12:3499-509|
|Calkins, Marcus J; Reddy, P Hemachandra (2011) Amyloid beta impairs mitochondrial anterograde transport and degenerates synapses in Alzheimer's disease neurons. Biochim Biophys Acta 1812:507-13|