Abstract

The requested spinning disk microscope will allow the core researchers at the University of Washington to advance research using live imaging technologies. The spinning disk microscope allows rapid sampling of fluorescent images in living specimens. Because the speed of image capture is increased on spinning disk systems, very rapid processes can be imaged at high resolution, including vesicular trafficking, changes in mitochondrial dynamics, changes in intracellular ion concentrations, fusion of membranes, cytoskeletal remodeling events, rapid movement of cells, etc. Minimizing light exposure is critical for many live applications to reduce potential phototoxicity. Spinning disk microscopes subject specimens to much lower light levels than conventional confocal microscopes, and are thus well suited for live imaging. Projects supported by the instrument include the understanding of developmental cell fate, which will have critical impact on our understanding of birth defects and other genetic diseases, the molecular basis of hearing loss, and a detailed study of the host-pathogen interactions leading to tuberculosis, a debilitating and lethal infection of children and adults.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR026826-01
Application #
7792162
Study Section
Special Emphasis Panel (ZRG1-IMST-A (30))
Program Officer
Levy, Abraham
Project Start
2010-05-06
Project End
2011-05-05
Budget Start
2010-05-06
Budget End
2011-05-05
Support Year
1
Fiscal Year
2010
Total Cost
$499,532
Indirect Cost

  Institution

Name
University of Washington
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Hailey, Dale W; Esterberg, Robert; Linbo, Tor H et al. (2017) Fluorescent aminoglycosides reveal intracellular trafficking routes in mechanosensory hair cells. J Clin Invest 127:472-486
Thomas, Eric D; Cruz, Ivan A; Hailey, Dale W et al. (2015) There and back again: development and regeneration of the zebrafish lateral line system. Wiley Interdiscip Rev Dev Biol 4:1-16
Cruz, Ivan A; Kappedal, Ryan; Mackenzie, Scott M et al. (2015) Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance. Dev Biol 402:229-38
Bouldin, Cortney M; Snelson, Corey D; Farr 3rd, Gist H et al. (2014) Restricted expression of cdc25a in the tailbud is essential for formation of the zebrafish posterior body. Genes Dev 28:384-95
Stawicki, Tamara M; Owens, Kelly N; Linbo, Tor et al. (2014) The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity and function. Dis Model Mech 7:847-56
Esterberg, Robert; Hailey, Dale W; Rubel, Edwin W et al. (2014) ER-mitochondrial calcium flow underlies vulnerability of mechanosensory hair cells to damage. J Neurosci 34:9703-19
Gau, Philia; Poon, Jason; Ufret-Vincenty, Carmen et al. (2013) The zebrafish ortholog of TRPV1 is required for heat-induced locomotion. J Neurosci 33:5249-60
Thomas, Andrew J; Hailey, Dale W; Stawicki, Tamara M et al. (2013) Functional mechanotransduction is required for cisplatin-induced hair cell death in the zebrafish lateral line. J Neurosci 33:4405-14
Esterberg, Robert; Hailey, Dale W; Coffin, Allison B et al. (2013) Disruption of intracellular calcium regulation is integral to aminoglycoside-induced hair cell death. J Neurosci 33:7513-25
Hailey, Dale W; Roberts, Brock; Owens, Kelly N et al. (2012) Loss of Slc4a1b chloride/bicarbonate exchanger function protects mechanosensory hair cells from aminoglycoside damage in the zebrafish mutant persephone. PLoS Genet 8:e1002971

Showing the most recent 10 out of 11 publications