In order to address the overwhelming demand for multi-color flow cytometry cell sorting, LIAI is requesting funds for the replacement of a Becton Dickinson (BD;BD Biosciences San Jose, CA) FACS Vantage SE that the manufacturer will no longer support, with a 9-color and 2 scatter parameter digital BD FACSAria II cell sorter. The facility already operates at near maximum capacity due to incredible demand from an institute devoted to the study of the immune system, and only by replacement of this instrument with a faster and more capable unit will the facility be able to fully support the research program. The requested instrument has been chosen because it can analyze up to 11 parameters simultaneously and will significantly enhance the ability to evaluate numerous phenotypic parameters of a cell concurrently, as well as greatly increased sample throughput/efficiency over the current instrument. Importantly, this high- speed instrument will maximize the opportunity to sort from high cell count samples and capture rare cell populations that are often technically challenging to collect. With a large group of extraordinarily high volume users, replacement of the current instrument will also leverage the ability to fully segregate conventional samples from biohazardous samples that will be run on an existing unit in a purpose built room and hood combination. This segregation will greatly increase safety and scheduling efficiencies that in total are expected to increase the facilities sorting capacity by nearly 1,000 hours per year;an urgent need in the support of over 100 NIH-supported investigators.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-CB-J (30))
Program Officer
Levy, Abraham
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
La Jolla Institute
La Jolla
United States
Zip Code
Schmiedel, Benjamin J; Singh, Divya; Madrigal, Ariel et al. (2018) Impact of Genetic Polymorphisms on Human Immune Cell Gene Expression. Cell 175:1701-1715.e16
Seo, Goo-Young; Shui, Jr-Wen; Takahashi, Daisuke et al. (2018) LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection. Cell Host Microbe 24:249-260.e4
Mehta, A K; Doherty, T; Broide, D et al. (2018) Tumor necrosis factor family member LIGHT acts with IL-1? and TGF-? to promote airway remodeling during rhinovirus infection. Allergy 73:1415-1424
Winkels, Holger; Ehinger, Erik; Vassallo, Melanie et al. (2018) Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry. Circ Res 122:1675-1688
Li, Qian; Li, Dulei; Zhang, Xian et al. (2018) E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity 48:258-270.e5
Burel, Julie G; Lindestam Arlehamn, Cecilia S; Khan, Nabeela et al. (2018) Transcriptomic Analysis of CD4+ T Cells Reveals Novel Immune Signatures of Latent Tuberculosis. J Immunol 200:3283-3290
Havenar-Daughton, Colin; Abbott, Robert K; Schief, William R et al. (2018) When designing vaccines, consider the starting material: the human B cell repertoire. Curr Opin Immunol 53:209-216
Grifoni, Alba; Costa-Ramos, Priscilla; Pham, John et al. (2018) Cutting Edge: Transcriptional Profiling Reveals Multifunctional and Cytotoxic Antiviral Responses of Zika Virus-Specific CD8+ T Cells. J Immunol 201:3487-3491
Chandra, Shilpi; Gray, James; Kiosses, William B et al. (2018) Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279
Havenar-Daughton, Colin; Sarkar, Anita; Kulp, Daniel W et al. (2018) The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen. Sci Transl Med 10:

Showing the most recent 10 out of 88 publications