This training grant will continue to provide support for graduate student studying in the area of molecular biophysics. The support provided through this mechanism serves to encourage students entering the University of California at Berkeley to choose biophysics/structural biology as their area of specialization for graduate study, and to increase the options for them. It also provides resources for the Structural Biology Seminar program, bringing in prominent workers in this field to present their work and meet with students and faculty. The students take a small number of core courses, which are centered, in contemporary problem in molecular biophysics, and the methods for analysis of such systems. These are complemented with other courses to fill in background and strengthen understanding in both systems of interest and their context in the complex cellular environment. Research projects span many areas including: determining three dimensional structures of proteins and nucleic acids in 3D crystals, 2D crystals, membranes and in solution; determining mechanism of enzyme catalysis and the relationship of protein dynamics to catalysis; the higher order structure of DNA in cells and how it is maintained and regulated; the folding of complex RNAs, and RNA protein interactions; the structure of biological membranes and how they influence cell-cell interactions; the folding pathways of proteins and the basis for protein stability; theoretical methods for understanding protein folding and networks of interacting proteins in cells; the use of mass spectroscopy and other methods for proteomics. For trainees who are entering graduate students the first year program includes rotations and selection of a research director, starting research work, doing course work, training as an instructor (teaching assistant), and attending seminars, particularly the weekly Structural Biology Seminar. In the second year research becomes a major focus, although coursework and teaching continue, as does attending seminars. For trainees who are supported later in their graduate stay a majority of time is spent in research, but regular attendance at seminars is expected. Courses in the fast two years include core courses in MCB and Chemistry on systems and methods for biophysical analysis, as well as background courses in fundamentals of physics, chemistry and biology selected to complement the student's undergraduate background. Special courses in techniques, such as x-ray crystallography and NMR, are offered, as are courses in specialized systems such as enzymes or nucleic acids.
Shah, Neel H; Amacher, Jeanine F; Nocka, Laura M et al. (2018) The Src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases. Crit Rev Biochem Mol Biol 53:535-563 |
Schöneberg, Johannes; Pavlin, Mark Remec; Yan, Shannon et al. (2018) ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science 362:1423-1428 |
Amacher, Jeanine F; Hobbs, Helen T; Cantor, Aaron C et al. (2018) Phosphorylation control of the ubiquitin ligase Cbl is conserved in choanoflagellates. Protein Sci 27:923-932 |
Del Mármol, Josefina; Rietmeijer, Robert A; Brohawn, Stephen G (2018) Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes. Methods Mol Biol 1684:129-150 |
Sasmal, Sukanya; Lincoff, James; Head-Gordon, Teresa (2017) Effect of a Paramagnetic Spin Label on the Intrinsically Disordered Peptide Ensemble of Amyloid-?. Biophys J 113:1002-1011 |
Mir, Mustafa; Reimer, Armando; Haines, Jenna E et al. (2017) Dense Bicoid hubs accentuate binding along the morphogen gradient. Genes Dev 31:1784-1794 |
Li, Bo; Tambe, Akshay; Aviran, Sharon et al. (2017) PROBer Provides a General Toolkit for Analyzing Sequencing-Based Toeprinting Assays. Cell Syst 4:568-574.e7 |
Nogales, Eva; Patel, Avinash B; Louder, Robert K (2017) Towards a mechanistic understanding of core promoter recognition from cryo-EM studies of human TFIID. Curr Opin Struct Biol 47:60-66 |
Harrington, Lucas B; Doxzen, Kevin W; Ma, Enbo et al. (2017) A Broad-Spectrum Inhibitor of CRISPR-Cas9. Cell 170:1224-1233.e15 |
Horitani, Masaki; Offenbacher, Adam R; Carr, Cody A Marcus et al. (2017) 13C ENDOR Spectroscopy of Lipoxygenase-Substrate Complexes Reveals the Structural Basis for C-H Activation by Tunneling. J Am Chem Soc 139:1984-1997 |
Showing the most recent 10 out of 99 publications