Human embryonic stem cells (hESCs), induced pluripotent stem cells (iPSCs), and adult stem cells are increasingly being investigated for treating many diseases, tissue engineering, regenerative medicine, and the development of disease specific tissue models for genomic analysis and in vitro drug screening. However, it is difficult to precisely control the behavior of stem cells, since environmental conditions for self-renewal and differentiation are not well understood, yet precise control is essential to realizing all of these downstream benefits. Graduate education has traditionally been successful in educating students in either engineering or biology, but the disparate nature of the scientific and engineering backgrounds necessary to move this field forward requires novel methodology in education is necessary for success. The University of California at Berkeley proposes an interdisciplinary training program in Stem Cell Engineering, its application to the treatment of disease, and the legal and ethical issues surrounding the study and use of stem cells. With the involvement and support of the Berkeley Stem Cell Center, Bioengineering Department, and Molecular and Cell Biology Department, we have designed a program to support the education and training of predoctoral fellows in issues relating to stem cell engineering. This newly created discipline represents the convergence of the biological and physical sciences, engineering, and ethics and law. The primary objectives of this program will be to formally organize the structure and scope of new training opportunities in this emerging and rapidly expanding discipline, to dissolve traditional academic barriers to interdisciplinary graduate science education, and to provide strong research training in academia and industry. As part of these efforts, we have developed a new Stem Cell Engineering curriculum, a seminar series, an annual retreat, interdisciplinary research, and an industrial internship experience. The result will be highly effective young scientists trained to work at the interface of biology and engineering and within a very timely area of biomedical research.

Public Health Relevance

The primary objective of this Stem Cell Engineering (SCE) Training Program will be to formally build and institute a structure and scope for research and classroom training opportunities in this emerging and rapidly expanding discipline, and reduce traditional academic barriers to interdisciplinary graduate science and engineering education.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Institutional National Research Service Award (T32)
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Training and Workforce Development Subcommittee - D (TWD)
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Gerratana, Barbara
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University of California Berkeley
Engineering (All Types)
Biomed Engr/Col Engr/Engr Sta
United States
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Mohrin, Mary; Widjaja, Andrew; Liu, Yufei et al. (2018) The mitochondrial unfolded protein response is activated upon hematopoietic stem cell exit from quiescence. Aging Cell 17:e12756
Lin, Jung-Ming G; Kang, Chi-Chih; Zhou, Yun et al. (2018) Linking invasive motility to protein expression in single tumor cells. Lab Chip 18:371-384
Miyano, Masaru; Sayaman, Rosalyn W; Stoiber, Marcus H et al. (2017) Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells. Aging (Albany NY) 9:2026-2051
Loskill, Peter; Sezhian, Thiagarajan; Tharp, Kevin M et al. (2017) WAT-on-a-chip: a physiologically relevant microfluidic system incorporating white adipose tissue. Lab Chip 17:1645-1654
Fletcher, Russell B; Das, Diya; Gadye, Levi et al. (2017) Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution. Cell Stem Cell 20:817-830.e8
Epstein, Benjamin E; Schaffer, David V (2017) Combining Engineered Nucleases with Adeno-associated Viral Vectors for Therapeutic Gene Editing. Adv Exp Med Biol 1016:29-42
Gadye, Levi; Das, Diya; Sanchez, Michael A et al. (2017) Injury Activates Transient Olfactory Stem Cell States with Diverse Lineage Capacities. Cell Stem Cell 21:775-790.e9
Guillou, Lionel; Dahl, Joanna B; Lin, Jung-Ming G et al. (2016) Measuring Cell Viscoelastic Properties Using a Microfluidic Extensional Flow Device. Biophys J 111:2039-2050
Gaj, Thomas; Epstein, Benjamin E; Schaffer, David V (2016) Genome Engineering Using Adeno-associated Virus: Basic and Clinical Research Applications. Mol Ther 24:458-64
Dahl, Joanna B; Lin, Jung-Ming G; Muller, Susan J et al. (2015) Microfluidic Strategies for Understanding the Mechanics of Cells and Cell-Mimetic Systems. Annu Rev Chem Biomol Eng 6:293-317

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