This is a renewal proposal for a training program that has been active for the past 25 years. This training program is associated with the Genome Sciences Department within the Life Sciences Division at the Lawrence Berkeley National Laboratory (LBNL). LBNL is affiliated with the University of California at Berkeley and physically its laboratories are contiguous with the Berkeley campus. The previous training program heavily emphasized the use of structural as well as genetic approaches to studies of lipoproteins and cardiovascular biology. The present submission of the program, in addition to these molecular approaches, emphasizes an increased use of genomic technologies including sequence analysis, expression profiling and model organisms to address a variety of issues of basic relevance to cardiovascular biology and lipoprotein metabolism. Approaches that will be utilized in these studies include: molecular biological studies of transgenic and gene knockout mice, site specific mutagenesis, genome sequencing, informatic analysis of sequence and expression profiling data and the manipulations of the genomes of mice, Drosophila and C. elegans. The varied expertise of the training faculty forms the basis for our multi-disciplinary training program. Because the training program has been expanded, the name of our training program has been changed to """"""""Genomic Approaches to Cardiovascular Disorders"""""""" to reflect this new orientation. Our program provides strong interdisciplinary training in cellular, molecular, biophysical, computational, genetic and genomic aspects of lipoprotein metabolism, atherogenesis and cardiovascular biology. In addition, there is extensive emphasis on genomic approaches to study gene regulation with a focus on the cardiovascular system. The Cross-disciplinary training the fellows receive serves as an excellent background for the trainees to develop independent research programs. Trainees completing the program have numerous career opportunities including academia, research in non-profit research laboratories, and the pharmaceutical and biotechnology industries. Because of the trainees' broad background, it is our experience that they are actively recruited by both academia as well as by the flourishing San Francisco Bay area bio-technology industries.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Institutional National Research Service Award (T32)
Project #
5T32HL007279-28
Application #
6906423
Study Section
Special Emphasis Panel (ZHL1-CSR-M (F1))
Program Officer
Scott, Jane
Project Start
1982-07-01
Project End
2008-06-30
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
28
Fiscal Year
2005
Total Cost
$31,881
Indirect Cost
Name
Lawrence Berkeley National Laboratory
Department
Genetics
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
Carlton, Peter M; Farruggio, Alfonso P; Dernburg, Abby F (2006) A link between meiotic prophase progression and crossover control. PLoS Genet 2:e12
Lipatov, Mikhail; Lenkov, Kapa; Petrov, Dmitri A et al. (2005) Paucity of chimeric gene-transposable element transcripts in the Drosophila melanogaster genome. BMC Biol 3:24
Noonan, James P; Hofreiter, Michael; Smith, Doug et al. (2005) Genomic sequencing of Pleistocene cave bears. Science 309:597-9
Bergman, Casey M; Carlson, Joseph W; Celniker, Susan E (2005) Drosophila DNase I footprint database: a systematic genome annotation of transcription factor binding sites in the fruitfly, Drosophila melanogaster. Bioinformatics 21:1747-9
Pollard, Daniel A; Bergman, Casey M; Stoye, Jens et al. (2004) Benchmarking tools for the alignment of functional noncoding DNA. BMC Bioinformatics 5:6
Bergman, Casey M; Pfeiffer, Barret D; Rincon-Limas, Diego E et al. (2002) Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome. Genome Biol 3:RESEARCH0086
Kaminker, Joshua S; Bergman, Casey M; Kronmiller, Brent et al. (2002) The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective. Genome Biol 3:RESEARCH0084
Callow, M J; Stoltzfus, L J; Lawn, R M et al. (1994) Expression of human apolipoprotein B and assembly of lipoprotein(a) in transgenic mice. Proc Natl Acad Sci U S A 91:2130-4
McCall, M R; Nichols, A V; Morton, R E et al. (1993) Transformation of HepG2 nascent lipoproteins by LCAT: modulation by HepG2 d > 1.235 g/ml fraction. J Lipid Res 34:37-48
Hara, S; McCall, M R; Forte, T M (1993) Re-uptake of nascent low-density lipoproteins by HepG2 cells. Biochim Biophys Acta 1168:199-204

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