Abstract

Regions of the HIV envelope protein involved in T4 binding will be defined in direct and competition binding studies employing fragments of gp120 and T4 positive cells. We will also examine the mechanism of gp120/gp41 mediated cell fusion (1) by studying fusion of intact cells transfected with vectors expressing native and mutated forms of gp120/gp41 and (2) utilizing lipid vesicles reconstituted with HIV envelope proteins and envelope from other fusogenic viruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AI025721-03
Application #
3814670
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Buffalo
Department
Type
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260

  Publications

Dundr, M; Meier, U T; Lewis, N et al. (1997) A class of nonribosomal nucleolar components is located in chromosome periphery and in nucleolus-derived foci during anaphase and telophase. Chromosoma 105:407-17
Dundr, M; Leno, G H; Lewis, N et al. (1996) Location of the HIV-1 Rev protein during mitosis: inactivation of the nuclear export signal alters the pathway for postmitotic reentry into nucleoli. J Cell Sci 109 ( Pt 9):2239-51
Berkowitz, R D; Hammarskjold, M L; Helga-Maria, C et al. (1995) 5' regions of HIV-1 RNAs are not sufficient for encapsidation: implications for the HIV-1 packaging signal. Virology 212:718-23
Srinivasakumar, N; Hammarskjold, M L; Rekosh, D (1995) Characterization of deletion mutations in the capsid region of human immunodeficiency virus type 1 that affect particle formation and Gag-Pol precursor incorporation. J Virol 69:6106-14
Dundr, M; Leno, G H; Hammarskjold, M L et al. (1995) The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein. J Cell Sci 108 ( Pt 8):2811-23
Mak, J; Jiang, M; Wainberg, M A et al. (1994) Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles. J Virol 68:2065-72
Hammarskjold, M L; Li, H; Rekosh, D et al. (1994) Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron. J Virol 68:951-8
Bray, M; Prasad, S; Dubay, J W et al. (1994) A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent. Proc Natl Acad Sci U S A 91:1256-60
Smith, A J; Srinivasakumar, N; Hammarskjold, M L et al. (1993) Requirements for incorporation of Pr160gag-pol from human immunodeficiency virus type 1 into virus-like particles. J Virol 67:2266-75
Keefer, M C; Bonnez, W; Roberts Jr, N J et al. (1991) Human immunodeficiency virus (HIV-1) gp160-specific lymphocyte proliferative responses of mononuclear leukocytes from HIV-1 recombinant gp160 vaccine recipients. J Infect Dis 163:448-53

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