Core - Protein Production and Assay

Reinherz, Ellis L.

Abstract

Core B will produce recombinant gp120 in various forms for assay purposes and physical studies. Recombinant gp120 will be produced in a baculovirus/insect cell system which has been demonstrated to be successful. Core B will carry out assay procedures based on binding of gp120 to a version of CD4, both in cellfree and cell based assays. Finally, the core group will do experiments with live HIV in cell cultures to examine antiviral activities of test compounds generated at Procept, Inc.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Project--Cooperative Agreements (U01)
• Project #: 3U01AI027336-09S1
• Application #: 6099274
• Project Start: 1996-12-01
• Project End: 1998-09-30
• Support Year: 9
• Fiscal Year: 1998

Institution

• Name: Dana-Farber Cancer Institute
• DUNS #: 149617367
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02215

Publications

Ferrer, M; Harrison, S C (1999) Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J Virol 73:5795-802

Tan, K; Liu, J; Wang, J et al. (1997) Atomic structure of a thermostable subdomain of HIV-1 gp41. Proc Natl Acad Sci U S A 94:12303-8

Ferrer, M; Godbout, K L; Sullivan, B J et al. (1997) Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J Immunol Methods 210:215-25

Sakihama, T; Smolyar, A; Reinherz, E L (1995) Oligomerization of CD4 is required for stable binding to class II major histocompatibility complex proteins but not for interaction with human immunodeficiency virus gp120. Proc Natl Acad Sci U S A 92:6444-8

Sakihama, T; Smolyar, A; Reinherz, E L (1995) Molecular recognition of antigen involves lattice formation between CD4, MHC class II and TCR molecules. Immunol Today 16:581-7

Bell, K D; Ramilo, O; Vitetta, E S (1993) Combined use of an immunotoxin and cyclosporine to prevent both activated and quiescent peripheral blood T cells from producing type 1 human immunodeficiency virus. Proc Natl Acad Sci U S A 90:1411-5

Moebius, U; Pallai, P; Harrison, S C et al. (1993) Delineation of an extended surface contact area on human CD4 involved in class II major histocompatibility complex binding. Proc Natl Acad Sci U S A 90:8259-63

Moebius, U; Clayton, L K; Abraham, S et al. (1992) The human immunodeficiency virus gp120 binding site on CD4: delineation by quantitative equilibrium and kinetic binding studies of mutants in conjunction with a high-resolution CD4 atomic structure. J Exp Med 176:507-17

Moebius, U; Clayton, L K; Abraham, S et al. (1992) Human immunodeficiency virus gp120 binding C'C"" ridge of CD4 domain 1 is also involved in interaction with class II major histocompatibility complex molecules. Proc Natl Acad Sci U S A 89:12008-12

Ghetie, V; Wheeler, T; Scott, D et al. (1992) A CD4-derived peptide carrier blocks acute HIV-1 infection in vitro and binds to gp120 in the presence of Walter-Reed stage 1-6 HIV+ sera. AIDS Res Hum Retroviruses 8:1945-8