We propose to evaluate virus-load in lymph nodes and other tissues involved in the HIV-pathogenesis in vivo. Although quantitative cell cultures and plasma viremia are extremely useful indicators of the circulating virus in the blood, these assays do not assess the virus-burden locally in the affected tissues. Further, because of the increasing frequency of HIV-related lymphomas and the recognized significance of neurologic complications in these individuals, it is important to identify target cells and focal areas of HIV infection in various tissues. Our project therefore will focus on the quantitative assessment of HIV-infected cells by in situ molecular hybridization of mRNA, RNA/DNA PCR as a confirmatory test and immunohistochemical staining for the identification of cell types in situ. The proposal is also based on the fact that our ATEU has one of the largest patient population of AIDS-related lymphoma in the country and a significant number of specimens are already available from the ACTG-studies that have been completed or closed to accrual. This offers a unique opportunity to study sequential samples from clinically well-defined patients with distinct pathologic manifestations and to develop a useful clinical test on specimens that are routinely obtained for diagnosis and treatment of these patients.

Project Start
1998-01-01
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
12
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Southern California
Department
Type
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
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