Abstract

This Core will serve as a resource for Projects 1, 2, and 3. Peptides will be synthesized from Fmoc-protected amino acids using automated instrumentation or by manual methods. The scale of synthesis will range from 0.1 to 2 grams of each peptide. Synthetic preparations which are determined to be homogeneous will be provided to leaders of Projects 1, 2 and 3 for use in in vitro and in vivo studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AI031696-04
Application #
3747258
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Dong, H; Courchesne, W (1998) A novel quantitative mating assay for the fungal pathogen Cryptococcus neoformans provides insight into signalling pathways responding to nutrients and temperature. Microbiology 144 ( Pt 6):1691-7
Hristova, K; White, S H (1998) Determination of the hydrocarbon core structure of fluid dioleoylphosphocholine (DOPC) bilayers by x-ray diffraction using specific bromination of the double-bonds: effect of hydration. Biophys J 74:2419-33
Wimley, W C; Hristova, K; Ladokhin, A S et al. (1998) Folding of beta-sheet membrane proteins: a hydrophobic hexapeptide model. J Mol Biol 277:1091-110
Belay, T; Cherniak, R; Kozel, T R et al. (1997) Reactivity patterns and epitope specificities of anti-Cryptococcus neoformans monoclonal antibodies by enzyme-linked immunosorbent assay and dot enzyme assay. Infect Immun 65:718-28
Hristova, K; Selsted, M E; White, S H (1997) Critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins. J Biol Chem 272:24224-33
Savoy, A C; Lupan, D M; Manalo, P B et al. (1997) Acute lethal toxicity following passive immunization for treatment of murine cryptococcosis. Infect Immun 65:1800-7
Selsted, M E (1997) HPLC methods for purification of antimicrobial peptides. Methods Mol Biol 78:17-33
Belay, T; Cherniak, R; O'Neill, E B et al. (1996) Serotyping of Cryptococcus neoformans by dot enzyme assay. J Clin Microbiol 34:466-70
Hristova, K; Selsted, M E; White, S H (1996) Interactions of monomeric rabbit neutrophil defensins with bilayers: comparison with dimeric human defensin HNP-2. Biochemistry 35:11888-94
Kozel, T R (1996) Activation of the complement system by pathogenic fungi. Clin Microbiol Rev 9:34-46

Showing the most recent 10 out of 29 publications