Abstract

The purpose of the core is to provide HLA typing for class I and class II alleles for all projects. The typing will be performed in the Immunogenetics Laboratory of Georgetown University Medical School. Five hundred individuals will be HLA type over the five year period. Standard microcytoxicity will be used to determine class I specificities using locally prepared typing trays specially designed to identify HLA specificities in individuals of African ancestry. A polymerase chain reaction based DNA typing method, Sequence Specific Primers (PCR-SSP), will be used to determine class II alleles. The technology will be transferred to Cameroon during the fourth year through a training workshop. We will correlate immune responses to defined P. falciparum vaccine candidate antigens with HLA type to determine whether HLA affects the specificity and/or rate of development of protective immunity. A Cameroonian collaborator will travel to the US during year one to learn how to collect and preserve samples for HLA typing. In year three, core personnel will travel to Cameroon to consult with researchers and help prepare for the training workshop to be held in year 4.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AI035839-05
Application #
6099801
Study Section
Project Start
1998-04-01
Project End
2000-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Chang, Sandra P; Kayatani, Alexander K K; Terrientes, Zilka I et al. (2010) Shift in epitope dominance of IgM and IgG responses to Plasmodium falciparum MSP1 block 4. Malar J 9:14
Thévenon, Audrey Davidson; Zhou, James A; Megnekou, Rosette et al. (2010) Elevated levels of soluble TNF receptors 1 and 2 correlate with Plasmodium falciparum parasitemia in pregnant women: potential markers for malaria-associated inflammation. J Immunol 185:7115-22
Taylor, Diane Wallace; Zhou, Aniong; Marsillio, Lauren E et al. (2004) Antibodies that inhibit binding of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A and to the C terminus of merozoite surface protein 1 correlate with reduced placental malaria in Cameroonian women. Infect Immun 72:1603-7
Johnson, Armead H; Leke, Rose G F; Mendell, Nancy R et al. (2004) Human leukocyte antigen class II alleles influence levels of antibodies to the Plasmodium falciparum asexual-stage apical membrane antigen 1 but not to merozoite surface antigen 2 and merozoite surface protein 1. Infect Immun 72:2762-71
Xi, Guoling; Leke, Rose G F; Thuita, Lucy W et al. (2003) Congenital exposure to Plasmodium falciparum antigens: prevalence and antigenic specificity of in utero-produced antimalarial immunoglobulin M antibodies. Infect Immun 71:1242-6
Zhou, Ainong; Megnekou, Rosette; Leke, Robert et al. (2002) Prevalence of Plasmodium falciparum infection in pregnant Cameroonian women. Am J Trop Med Hyg 67:566-70
Staalsoe, T; Megnekou, R; Fievet, N et al. (2001) Acquisition and decay of antibodies to pregnancy-associated variant antigens on the surface of Plasmodium falciparum-infected erythrocytes that protect against placental parasitemia. J Infect Dis 184:618-26
O'Neil-Dunne, I; Achur, R N; Agbor-Enoh, S T et al. (2001) Gravidity-dependent production of antibodies that inhibit binding of Plasmodium falciparum-infected erythrocytes to placental chondroitin sulfate proteoglycan during pregnancy. Infect Immun 69:7487-92
Johnson, A; Leke, R; Harun, L et al. (2000) Interaction of HLA and age on levels of antibody to Plasmodium falciparum rhoptry-associated proteins 1 and 2. Infect Immun 68:2231-6
Ellis, J M; Mack, S J; Leke, R F et al. (2000) Diversity is demonstrated in class I HLA-A and HLA-B alleles in Cameroon, Africa: description of HLA-A*03012, *2612, *3006 and HLA-B*1403, *4016, *4703. Tissue Antigens 56:291-302

Showing the most recent 10 out of 11 publications