Abstract

The larval stage of Taenia solium is responsible for neurological morbidity and mortality in humans, and important economical loses because of porcine infection. On the basis of previous successful cestode vaccines, we propose to develop and optimize a vaccine against T. solium. Primarily using Excretory/Secretory antigens from T. solium oncospheres. A key element in this design is the availability of a novel intramuscular pig infection model (IMOA) developed by our group, that is reproducible, easy to perform, and permits to establish several infection points in each animal. The project aims to fractionate and biochemically characterize E/S antigens and determine the ability of each fraction to protect pigs from oncosphere challenge using the IMOA. In vitro protection will be tested by using the immune complement killing assay. Recombinant proteins will be produced from protective antigens, and the best single or combination of recombinants will be used as a vaccine to determine its ability to protect in a T. solium-endemic, sentinel pigs, litters born to immunized dams and seronegative pigs. Under endemic conditions, a vaccine against porcine Cysticerosis would assist in preventing the transmission of T. solium by blocking larval stage development and could serve as an integral part of Cysticerosis control.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
2U01AI035894-06
Application #
6223839
Study Section
Special Emphasis Panel (ZAI1-MSQ-M (M1))
Project Start
1999-09-01
Project End
2004-08-31
Budget Start
Budget End
Support Year
6
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Gonzales, Isidro; Miranda, J Jaime; Rodriguez, Silvia et al. (2015) Seizures, cysticercosis and rural-to-urban migration: the PERU MIGRANT study. Trop Med Int Health 20:546-52
Arriola, Carmen S; Gonzalez, Armando E; Gomez-Puerta, Luis A et al. (2014) New insights in cysticercosis transmission. PLoS Negl Trop Dis 8:e3247
Gavidia, Cesar M; Verastegui, Manuela R; Garcia, Hector H et al. (2013) Relationship between serum antibodies and Taenia solium larvae burden in pigs raised in field conditions. PLoS Negl Trop Dis 7:e2192
O'Neal, Seth E; Moyano, Luz M; Ayvar, Viterbo et al. (2012) Geographic correlation between tapeworm carriers and heavily infected cysticercotic pigs. PLoS Negl Trop Dis 6:e1953
Gavidia, Cesar M; Gonzalez, Armando E; Barron, Eduardo A et al. (2010) Evaluation of oxfendazole, praziquantel and albendazole against cystic echinococcosis: a randomized clinical trial in naturally infected sheep. PLoS Negl Trop Dis 4:e616
Uddin, Jasim; Gonzalez, Armando E; Gilman, Robert H et al. (2010) Mechanisms regulating monocyte CXCL8 secretion in neurocysticercosis and the effect of antiparasitic therapy. J Immunol 185:4478-84
Sheen, Patricia; Ferrer, Patricia; Gilman, Robert H et al. (2009) Effect of pyrazinamidase activity on pyrazinamide resistance in Mycobacterium tuberculosis. Tuberculosis (Edinb) 89:109-13
Lescano, Andres G; Garcia, Hector H; Gilman, Robert H et al. (2009) Taenia solium cysticercosis hotspots surrounding tapeworm carriers: clustering on human seroprevalence but not on seizures. PLoS Negl Trop Dis 3:e371
Villarán, Manuel V; Montano, Silvia M; Gonzalvez, Guillermo et al. (2009) Epilepsy and neurocysticercosis: an incidence study in a Peruvian rural population. Neuroepidemiology 33:25-31
Gavidia, Cesar M; Gonzalez, Armando E; Lopera, Luis et al. (2009) Evaluation of nitazoxanide and oxfendazole efficacy against cystic echinococcosis in naturally infected sheep. Am J Trop Med Hyg 80:367-72

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