The overall aim of this application is to establish a Tissue Analysis and Technology Core (TATC) in support of the Multi-disciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network as described in RFA-DK-07-003. The MAPP Research Network will utilize a multi-site and multi-disciplinary approach to provide new insights into the pathogenesis and treatment of Chronic Pelvic Pain Syndromes, and establish a new and valuable biorepository resource for current and future research efforts. In response to the RFA, the TATC will interact with all research entities in the MAPP Network and provide expertise and resources in the areas of: 1) Biorepository Collection, Management and Distribution for all body fluid and tissue specimens generated by the MAPP Discovery Sites, and the generation of specialized assay platforms from these specimens such as protein and/or tissue arrays, DMA or RNA extractions as needed for Discovery Site efforts or the Ancillary Projects Program;2) Histopathology and Tissue Morphometry including diagnostic interpretation, paraffin processing and microtomy, cryomicrotomy, laser capture microdissection, immunohistochemistry, and computer-assisted imaging/ image analysis;3) Proteomics/ Metabolomics by providing a state-of-the-art centralized mass spectrometry facility to assist with proper collection and handling of specimens, consultative assistance for proteomic and/or metabolomics studies, and performance of a wide variety of assays, including two-dimensional gel- and chromatography-based proteome profiling, protein arrays, cytokine arrays, multiplexed ELISA, RT-PCR, infrared imaging, mass spectrometry and NMR-based targeted and non-targeted metabolite profiling, pharmacokinetic studies, oxidative stress assessment, and mass spectrometry analyses;and 4) Genomics/ Genotyping by providing consultative assistance and a state-of-the-art genomics facility to assist with advanced genotyping techniques. It is our underlying hypothesis that the quality, reliability and applicability of biological research on pelvic pain syndromes can only be guaranteed through the use of high quality non-biased patient specimens that have been handled, processed, analyzed and interpreted in a uniform manner. The MAPP Research Network will be the largest research initiative of its kind for chronic pelvic pain syndrome, a common but understudied condition in men and women. The establishment of a Tissue Analysis and Technology Core Facility is crucial to the success of this research program.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Project--Cooperative Agreements (U01)
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Special Emphasis Panel (ZDK1-GRB-G (M1))
Program Officer
Mullins, Christopher V
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University of Colorado Denver
Schools of Medicine
United States
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Sutcliffe, Siobhan; Jemielita, Thomas; Lai, H Henry et al. (2018) A Case-Crossover Study of Urological Chronic Pelvic Pain Syndrome Flare Triggers in the MAPP Research Network. J Urol 199:1245-1251
Clemens, J Quentin; Stephens-Shields, Alisa; Naliboff, Bruce D et al. (2018) Correlates of Health Care Seeking Activities in Patients with Urological Chronic Pelvic Pain Syndromes: Findings from the MAPP Cohort. J Urol 200:136-140
Schrepf, Andrew; Naliboff, Bruce; Williams, David A et al. (2018) Adverse Childhood Experiences and Symptoms of Urologic Chronic Pelvic Pain Syndrome: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Study. Ann Behav Med 52:865-877
Naliboff, Bruce D; Stephens, Alisa J; Lai, H Henry et al. (2017) Clinical and Psychosocial Predictors of Urological Chronic Pelvic Pain Symptom Change in 1 Year: A Prospective Study from the MAPP Research Network. J Urol 198:848-857
Kutch, Jason J; Labus, Jennifer S; Harris, Richard E et al. (2017) Resting-state functional connectivity predicts longitudinal pain symptom change in urologic chronic pelvic pain syndrome: a MAPP network study. Pain 158:1069-1082
Kutch, Jason J; Ichesco, Eric; Hampson, Johnson P et al. (2017) Brain signature and functional impact of centralized pain: a multidisciplinary approach to the study of chronic pelvic pain (MAPP) network study. Pain 158:1979-1991
Lai, H Henry; Jemielita, Thomas; Sutcliffe, Siobhan et al. (2017) Characterization of Whole Body Pain in Urological Chronic Pelvic Pain Syndrome at Baseline: A MAPP Research Network Study. J Urol 198:622-631
Dagher, Adelle; Curatolo, Adam; Sachdev, Monisha et al. (2017) Identification of novel non-invasive biomarkers of urinary chronic pelvic pain syndrome: findings from the Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network. BJU Int 120:130-142
Nickel, J Curtis; Stephens, Alisa; Landis, J Richard et al. (2016) Assessment of the Lower Urinary Tract Microbiota during Symptom Flare in Women with Urologic Chronic Pelvic Pain Syndrome: A MAPP Network Study. J Urol 195:356-62
Griffith, James W; Stephens-Shields, Alisa J; Hou, Xiaoling et al. (2016) Pain and Urinary Symptoms Should Not be Combined into a Single Score: Psychometric Findings from the MAPP Research Network. J Urol 195:949-54

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