Abstract

Public Health Relevance

The proposed studies on embryonic and reprogrammed stem cells will reveal parts of the complex mechanisms controlling cell fate, including those underlying self renewal, differentiation, and development of cancer. The results of our research will provide more efficient molecular tools allowing precise control over cell programming and reprogramming. The images and concepts that we produce will have immediate impact on regenerative medicine as well as stem cell and cancer research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01GM094614-01
Application #
8153362
Study Section
Special Emphasis Panel (ZGM1-CBB-0 (BC))
Project Start
2010-09-30
Project End
2015-06-30
Budget Start
2010-09-30
Budget End
2011-06-30
Support Year
1
Fiscal Year
2010
Total Cost
$238,628
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Miyaoka, Yuichiro; Mayerl, Steven J; Chan, Amanda H et al. (2018) Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR. Methods Mol Biol 1768:349-362
Cvoro, Aleksandra; Bajic, Aleksandar; Zhang, Aijun et al. (2016) Ligand Independent and Subtype-Selective Actions of Thyroid Hormone Receptors in Human Adipose Derived Stem Cells. PLoS One 11:e0164407
Benod, Cindy; Villagomez, Rosa; Webb, Paul (2016) TLX: An elusive receptor. J Steroid Biochem Mol Biol 157:41-7
Proudfoot, Andrew; Axelrod, Herbert L; Geralt, Michael et al. (2016) Dlx5 Homeodomain:DNA Complex: Structure, Binding and Effect of Mutations Related to Split Hand and Foot Malformation Syndrome. J Mol Biol 428:1130-1141
Kime, Cody; Mandegar, Mohammad A; Srivastava, Deepak et al. (2016) Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells. Curr Protoc Hum Genet 88:Unit 21.4
Hayashi, Yohei; Caboni, Laura; Das, Debanu et al. (2015) Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells. Proc Natl Acad Sci U S A 112:4666-71
Cvoro, Aleksandra; Devito, Liani; Milton, Flora A et al. (2015) A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells. Stem Cells 33:416-28
Carruthers, Carl W; Suh, Ji Ho; Gustafsson, Jan-Ake et al. (2015) Phosphorylation of glucocorticoid receptor tau1c transactivation domain enhances binding to CREB binding protein (CBP) TAZ2. Biochem Biophys Res Commun 457:119-23
Sablin, Elena P; Blind, Raymond D; Uthayaruban, Rubatharshini et al. (2015) Structure of Liver Receptor Homolog-1 (NR5A2) with PIP3 hormone bound in the ligand binding pocket. J Struct Biol 192:342-348
Spencer, C Ian; Baba, Shiro; Nakamura, Kenta et al. (2014) Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia. Stem Cell Reports 3:269-81

Showing the most recent 10 out of 18 publications