- Core B - BioCore Core B provides the resources and expertise to process specimens for the hematological, neuroendocrine, glucoregulatory, metabolic and cytokine indices needed for the research projects. The Core has the requisite infrastructure and oversight capabilities to ensure reliable and high quality data are generated in a high throughput manner with centralized testing at single sites. Specifically, the Core works with the 3 clinician- researchers who supervise nursing staff acquiring blood, urine and saliva specimens at the 3 regional Clinical Research Units. The BioCore then coordinates many tests at two CAP and CLIA-certified clinical laboratories for evaluating glucoregulation and lipid metabolism, as well as neuroendocrine assays, including catecholamines via LC/MS at the Endocrine Services unit of the UW-CTR. Some analytes are determined at labs that specialize in specific methods, such as dried blood spots (DBS) for the Retention-Early Warning project, run at the CLIA-CAP accredited Clinical Laboratories of the University of Washington School of Medicine. Core B also conducts assays, including multi-cytokine arrays using an electrochemiluminescence platform. Core B receives the saliva specimens for the Daily Stress project, monitors shipment integrity of the specimens upon receipt, and oversees the assay of salivary cortisol. To fulfill these functions, the Core is the primary liaison with project investigators and with medical directors at the testing labs, including Meriter Labs in Madison, WI and ARUP in Salt Lake City, UT. It has its own biochemical facilities for safe processing, including certified biosafety cabinets and fume hoods, follows Biosafety Level-2 protocols, and has the requisite number of ultracold freezers for long-term storage of archival aliquots. The scientific expertise of the Core staff positions MIDUS to be able to add innovative indices, such as ones employed in the Gene Expression project. It has enabled us to refine and validate more sensitive assays with Lower Limits of Detection (e.g., reducing the LLOD for urinary epinephrine by 1000-fold). In addition to assays and diagnostic testing, the Core is responsible for creating the data files of initial test results, which employ a distinct study code to protect participant confidentiality. In keeping with this record-keeping role, the Core serves as the repository for all original paper records of test results. It is responsible for all aspects of quality assurance, including monitoring the integrity of shipped specimens, quality control testing of assay reliability over time, and determines the need for any retesting of specimens, as well as oversees the 24/7 surveillance of our archival specimen repository in ultracold freezers. Finally, the Core has a pedagogical role. It generates norms for MIDUS test results to serve as a reference for investigators. In this educational capacity, the Core Director also provides instruction, giving workshops on specific biomarkers at the MIDUS meetings and consults with investigators on interpreting the meaning of biomarkers. The effectiveness and past success of the Core are evinced by 122 publications using MIDUS biomarker data, 47% of which were published in the last two years.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AG051426-01A1
Application #
9188965
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2016-07-19
Budget End
2017-06-30
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Delaney, Rebecca K; Turiano, Nicholas A; Strough, JoNell (2018) Living longer with help from others: Seeking advice lowers mortality risk. J Health Psychol 23:1590-1597
Chiang, Jessica J; Chen, Edith; Miller, Gregory E (2018) Midlife Self-Reported Social Support as a Buffer Against Premature Mortality Risks Associated with Childhood Abuse. Nat Hum Behav 2:261-268
Murdock, Kyle W; LeRoy, Angie S; Fagundes, Christopher P (2018) Inhibition is associated with metabolic syndrome and depression through inflammation. Stress Health 34:457-461
Danielson, Ramona; Sanders, Gregory F (2018) An effective measure of childhood adversity that is valid with older adults. Child Abuse Negl 82:156-167
Krueger, Robert F; Kotov, Roman; Watson, David et al. (2018) Progress in achieving quantitative classification of psychopathology. World Psychiatry 17:282-293
Piazza, Jennifer R; Stawski, Robert S; Sheffler, Julia L (2018) Age, Daily Stress Processes, and Allostatic Load: A Longitudinal Study. J Aging Health :898264318788493
Andersson, Matthew A (2018) A Discordance Weighting Approach Estimating Occupational and Income Returns to Education. Twin Res Hum Genet 21:191-202
Kong, Jooyoung; Moorman, Sara M; Martire, Lynn M et al. (2018) The Role of Current Family Relationships in Associations between Childhood Abuse and Adult Psychological Functioning. J Gerontol B Psychol Sci Soc Sci :
Beam, Christopher R; Marcus, Katherine; Turkheimer, Eric et al. (2018) Gender Differences in the Structure of Marital Quality. Behav Genet 48:209-223
Vittengl, Jeffrey R (2018) Mediation of the bidirectional relations between obesity and depression among women. Psychiatry Res 264:254-259

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