Human suffering and health care costs due to sequellae of STDs are escalating worldwide, including pelvic inflammatory disease, chronic pelvic pain, involuntarily infertility, and ectopic pregnancies. Project 5 will correlate clinical characteristics of T. vaginalis infections (including behavioral and demographic features as well as co-infections with other STD agents) with the presence or absence of dsRNA virus in T. vaginalis isolates. These two clinical T. vaginalis isolate types (i.e., with and without dsRNA virus) exist naturally and contribute to different outcomes in clinical demographic, and behavior parameters to be evaluated. Thus, as documented in Project 1, the virus provides a marker from which to carry out comparative clinical and adverse outcome studies.
Specific Aim 1 perform a comprehensive evaluation of T. vaginalis isolates with an without dsRNA virus and relate these data to various clinical parameters by a) examining characteristics (genitourinary symptoms and physical findings) between vaginitis caused by virus-harboring and virus-minus isolates in pregnant and non-pregnant women, b) evaluating behaviors, demographic features and partner history associated with the two isolate types of T. vaginalis, and c) establishing linkages between infection with the two isolate types and other STD agents.
Specific Aim 2 will evaluate the risk of adverse outcomes in women with STDs during pregnancy by a) examining infections by T. vaginalis with and without dsRNA virus and b) simultaneously examining and identifying other current infections (gonorrhea, chlamydia, bacterial vaginosis, group B, streptococcus, syphilis, HHV8, and M. genitalium).
Specific Aim 3 will facilitate collaborations with the other four Projects by a) providing fresh clinical T. vaginalis isolates from patients with trichomonosis for Project #1, b) coordinating identification and follow-up of pregnant women with the HHV8 Project #2 by collecting maternal samples and pregnancy outcome data, c) providing clinical data dn specimens for the Mycoplasma genitalium Project 3, and d) providing clinical expertise and additional infection information to Behavioral Project 4.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI045429-02
Application #
6344662
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2000
Total Cost
$129,053
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Zhang, Wenbo; Baseman, Joel B (2011) Transcriptional regulation of MG_149, an osmoinducible lipoprotein gene from Mycoplasma genitalium. Mol Microbiol 81:327-39
Zhang, Wenbo; Baseman, Joel B (2011) Transcriptional response of Mycoplasma genitalium to osmotic stress. Microbiology 157:548-56
Thurman, A R; Musatovova, O; Perdue, S et al. (2010) Mycoplasma genitalium symptoms, concordance and treatment in high-risk sexual dyads. Int J STD AIDS 21:177-83
Li, Linbo; Krishnan, Manickam; Baseman, Joel B et al. (2010) Molecular cloning, expression, and characterization of a Ca2+-dependent, membrane-associated nuclease of Mycoplasma genitalium. J Bacteriol 192:4876-84
Thurman, Andrea Ries; Holden, Alan E C; Shain, Rochelle N et al. (2009) Effect of acculturation on the acceptability of potential microbicides and sexual risk-taking. Sex Transm Dis 36:387-94
Kucknoor, Ashwini S; Mundodi, Vasanthakrishna; Alderete, Jf (2009) Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax. BMC Microbiol 9:58
Saikolappan, Sankaralingam; Sasindran, Smitha J; Yu, Hongwei D et al. (2009) The Mycoplasma genitalium MG_454 gene product resists killing by organic hydroperoxides. J Bacteriol 191:6675-82
Lama, A; Kucknoor, A; Mundodi, V et al. (2009) Glyceraldehyde-3-phosphate dehydrogenase is a surface-associated, fibronectin-binding protein of Trichomonas vaginalis. Infect Immun 77:2703-11
Balasubramanian, Sowmya; Kannan, T R; Hart, P John et al. (2009) Amino acid changes in elongation factor Tu of Mycoplasma pneumoniae and Mycoplasma genitalium influence fibronectin binding. Infect Immun 77:3533-41
Johnson, Coreen; Kannan, T R; Baseman, Joel B (2009) Characterization of a unique ADP-ribosyltransferase of Mycoplasma penetrans. Infect Immun 77:4362-70

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