The primary functions of Cell Therapy Core are to assist Projects 2-6 by making available critical equipment and technical expertise covering three distinct aspects of research support: optimal cryopreservation and storage of canine and human cells; characterization of canine transplantation products by flow cytometry; and stem cell enrichment from canine components. Specifically, this Core will: (A) store peripheral blood and/or serum specimens obtained from dogs and humans exposed to varying doses of irradiation as part of biodosimetry development Projects 2 and 3, and create a canine cord blood bank in support of Project 5. (B) Enrich for canine CD34+ cells to be used as the starting material for the ex vivo expanded myeloid progenitor cell studies described in Projects 4 and 5. As part of these projects the development of more optimized cryopreservation and storage conditions for these types of cells will also be necessary. (C) Analyze by flow cytometry all the various canine components used for transplantation as part of Projects 4-6. This proposed Cell Therapy Core represents an enhancement of an already existing Cell Processing resource that has considerable expertise in each of these areas. The existing resource manages several Research and Clinical Cell Repositories that contain a variety of cell types, encompassing over 50,000 different specimens stored in the vapor phase of liquid nitrogen freezers, including components used to support human therapeutic studies. The Processing Core has performed over 30 canine CD34+ cell enrichments, achieving excellent purities of >80% and yields of 50%. Finally, the Core specializes in flow cytometry analysis, and has established a database with over 3500 human clinical transplant components characterized for the content of hematopoietic stem cells (CD34), T-cell subsets (CDS, CD4, CDS, CD45RA), dendritic cells (CD14, BDCA-2), B-cells (CD19, CD22) and NK cell (CD56) subsets. These data form the basis for important correlative studies to investigate associations of these cell types with several clinical outcomes. In summary, Cell Therapy Core D will provide unique and critical services in support of Projects 2-6 described in this grant application, specifically optimal cryopreservation and storage, cell selection, and flow cytometry analysis, and will be able to provide those services in a more efficient and cost-effective manner.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Fred Hutchinson Cancer Research Center
United States
Zip Code
Nielsen, Christopher E; Wang, Xihai; Robinson, Robert J et al. (2014) Carcinogenic and inflammatory effects of plutonium-nitrate retention in an exposed nuclear worker and beagle dogs. Int J Radiat Biol 90:60-70
Venkataraman, G M; Geraghty, D; Fox, J et al. (2013) Canine DLA-79 gene: an improved typing method, identification of new alleles and its role in graft rejection and graft-versus-host disease. Tissue Antigens 81:204-11
Leko, Vid; Varnum-Finney, Barbara; Li, Hongzhe et al. (2012) SIRT1 is dispensable for function of hematopoietic stem cells in adult mice. Blood 119:1856-60
Nielsen, Christopher E; Wilson, Dulaney A; Brooks, Antone L et al. (2012) Microdistribution and long-term retention of 239Pu (NO3)4 in the respiratory tracts of an acutely exposed plutonium worker and experimental beagle dogs. Cancer Res 72:5529-36
Pogosova-Agadjanyan, Era L; Fan, Wenhong; Georges, George E et al. (2011) Identification of radiation-induced expression changes in nonimmortalized human T cells. Radiat Res 175:172-84
Ivey, Richard G; Moore, Heather D; Voytovich, Uliana J et al. (2011) Blood-based detection of radiation exposure in humans based on novel phospho-Smc1 ELISA. Radiat Res 175:266-81
Mielcarek, Marco; Storb, Rainer; Georges, George E et al. (2011) Mesenchymal stromal cells fail to prevent acute graft-versus-host disease and graft rejection after dog leukocyte antigen-haploidentical bone marrow transplantation. Biol Blood Marrow Transplant 17:214-25
Graves, Scott S; Stone, Diane M; Loretz, Carol et al. (2011) Antagonistic and agonistic anti-canine CD28 monoclonal antibodies: tools for allogeneic transplantation. Transplantation 91:833-40
Wilson, Dulaney A; Brigantic, Andrea; Morgan, William F (2011) The association of inbreeding with lung fibrosis incidence in Beagle dogs that inhaled 238PuO2 or 239PuO2. Radiat Res 176:781-6
Lee, Won Sik; Suzuki, Yasuhiro; Graves, Scott S et al. (2011) Canine bone marrow-derived mesenchymal stromal cells suppress alloreactive lymphocyte proliferation in vitro but fail to enhance engraftment in canine bone marrow transplantation. Biol Blood Marrow Transplant 17:465-75

Showing the most recent 10 out of 26 publications