Atopic dermatitis (AD) is a biphasic inflammatory skin disease characterized by an initial phase predominated? by Th2 cytokines which then shifts to a second, more chronic Th1 eczematous phase. Studies have shown? that dendritic cells (DC) are important for the pathogenesis of AD. DC are considered the most potent antigen? presenting cells (ARC) by virtue of their ability to bridge an innate immune response to an adaptive immune? response. The status of DC at the time of priming CD4 T cells can determine the type of immunity, either T? helper 1 (Th1) or Th2. Recently, we have demonstrated that, depending on the ability to produce IL-10, DC? can direct either Th1 or Th2 immune responses. IL-10, a pleiotropic cytokine produced by many different cell? types, inhibits antigen-specific activation and proliferation of T cells and ultimately leads to the termination of? inflammatory responses. Thus, regulation of IL-10 can dramatically alter immune responses. IL-4, a Th2? inducing cytokine, inhibits DC IL-10 expression and subsequently induces IL-12. Thus, DC exposed to IL-4? secrete more IL-12 and promote Th1 instead of Th2 differentiation. These data demonstrate a complex? cytokine regulatory network and indicate that the same cytokine has a distinct function modulating the immune? response via ARC. We hypothesize that the ability of DC to produce IL-10 contributes to the pathogenesis? of AD by directing the Th immune response. In early AD, DC are activated by allergen via toll-like receptors? (TLR) and produce both inflammatory and anti-inflammatory cytokines directing Th2 development by secreting? high IL-10 and as a result, low IL-12. As the disease progresses, the amount of IL-4 in the local environment? is elevated and as a consequence DC will produce less IL-10 leading to a Th1 response. We will test this? hypothesis in the current application by focusing on DC function and regulation of DC-mediated pathogenesis? of AD using both animal models and human patients. Our long-term goal is to have a better understanding of? AD pathogenesis mediated by DC, which will help us to design improved therapeutic tools in the future. To? achieve this goal, we propose three specific aims.
In Aim 1, we will investigate the role of TLR-mediated? activation of DC with the hypothesis that TLR on DC are critical to activate DC when an allergen is? encountered and for subsequent inflammation.
Aim 2 will study DC-mediated immunity using transgenic mice? that express a constitutively active form of State (StatGVT) as an AD model system. We hypothesize that? DC functions are altered in these mice because StatGVT mice have elevated levels of Th2 cytokines including? IL-4. The last Aim is devoted to studying DC function from AD patients by testing the wash fluid from the skin? lesion and blood samples collected from patients. Using both an animal model that develops spontaneous? AD-like symptoms as well as AD patients, we will obtain valuable information that will provide us a better? understanding of DC-mediated immunity in AD and other allergic diseases.
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