Background: While epidemiological studies have demonstrated an association between ambient particulate pollution and adverse health effect, uncertainties still exist concerning specific properties of particles that affect pathophysiological mechanisms. Often it has been very difficult to reproduce ambient conditions in a laboratory with sufficient doses to elicit effects. The development of ambient particulate concentrators has bridged this gap, effectively merging the ambient environment with the laboratory. Concentrated ambient particle experiments have linked real-world particulate matter to a host of health effects observed in human and animal exposure studies as well as in vitro.
Specific Aims : The objective of this 5-year project core, under the leadership of Dr Contantinos Sioutas, is to support project investigators and Center members who will be investigating the effects of ambient particulate matter on human health, including allergic inflammation. This core will provide chemically-characterized, concentrated ambient fine and ultrafine particle suspensions collected in downtown Los Angeles. The proposed project expands upon the NIEHS and US EPA funded research concerning the adverse health effects of particulate matter that has taken place at USC and UCLA over the past five years. Methods: Concentrated ambient fine and ultrafine particles will be collected by means of new and improved portable concentrators developed over the past five years by the University of Southern California, at a typical urban background site in downtown Los Angeles. The majority of the concentrated flow will be collected in aqueous suspension, immediately frozen with dry ice and transferred to UCLA for toxicity analysis. This procedure minimizes any possible chemical reactions in the suspension, maintaining its realworld characteristics. Aerosol characterization will include mass and chemistry measurements, including trace elements, inorganic ions, elemental/organic carbon, and PAHs. Relevance: The use of aerosol concentrators in conjunction with various in vitro and in vivo toxicity assays over the past five years has significantly improved researchers'understanding of health effects due to particles. Now that certain health endpoints have become the focus of experiments, the mechanisms leading to these endpoints can be investigated. Past studies have employed concentrators at various locations and seasons while emphasizing a variety of endpoints. By controlling these variables and enlarging the sample set, exploration into specific biological mechanisms behind observed health effects of particulate matter can begin.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI070453-05
Application #
8101285
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
5
Fiscal Year
2010
Total Cost
$156,378
Indirect Cost
Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Heber, David; Li, Zhaoping; Garcia-Lloret, Maria et al. (2014) Sulforaphane-rich broccoli sprout extract attenuates nasal allergic response to diesel exhaust particles. Food Funct 5:35-41
Li, Ning; Wang, Meiying; Barajas, Berenice et al. (2013) Nrf2 deficiency in dendritic cells enhances the adjuvant effect of ambient ultrafine particles on allergic sensitization. J Innate Immun 5:543-54
Ooi, Aik T; Ram, Sonal; Kuo, Alan et al. (2012) Identification of an interleukin 13-induced epigenetic signature in allergic airway inflammation. Am J Transl Res 4:219-28
Li, Ning; Nel, Andre E (2011) Feasibility of biomarker studies for engineered nanoparticles: what can be learned from air pollution research. J Occup Environ Med 53:S74-9
Li, Ning; Harkema, Jack R; Lewandowski, Ryan P et al. (2010) Ambient ultrafine particles provide a strong adjuvant effect in the secondary immune response: implication for traffic-related asthma flares. Am J Physiol Lung Cell Mol Physiol 299:L374-83
Kang, Xuedong; Li, Ning; Wang, Meiying et al. (2010) Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid. Proteomics 10:520-31
George, Saji; Pokhrel, Suman; Xia, Tian et al. (2010) Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping. ACS Nano 4:15-29
Tachdjian, Raffi; Al Khatib, Shadi; Schwinglshackl, Andreas et al. (2010) In vivo regulation of the allergic response by the IL-4 receptor alpha chain immunoreceptor tyrosine-based inhibitory motif. J Allergy Clin Immunol 125:1128-1136.e8
Kovochich, Michael; Espinasse, Benjamin; Auffan, Melanie et al. (2009) Comparative toxicity of C60 aggregates toward mammalian cells: role of tetrahydrofuran (THF) decomposition. Environ Sci Technol 43:6378-84
Nel, Andre E; M├Ądler, Lutz; Velegol, Darrell et al. (2009) Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater 8:543-57

Showing the most recent 10 out of 30 publications