The major objectives of the Tissue and Cell Processing Core are to provide to Program Investigators: (1) the highest quality processing of tissue specimens for paraffin-embedded, frozen- and plastic-embedded sections;(2) high fidelity primary cultures from tissue and cell specimens;(3) high throughput staining to facilitate stereology-based tissue quantitative histomorphometry (for tissues) and immunohistochemical staining (for tissues and cell cultures). The Tissue and Cell Processing Core is an essential component of this AADCRC application, facilitating carrying out the aims of the three Program proposals, each of which rely on varied histologic analyses of human and mouse tissues and in vitro studies of human and mouse nasal, tracheal and bronchial epithelial cells. The considerable experience of Core technical personnel and leadership facilitates the development of optimized protocols to achieve the objectives of each Project and also provides consistent high quality experimental materials which are often shared across Projects. The expertise of the Core is essential to optimizing yield from human and specific mouse tissue samples which are difficult to obtain and prohibitive to replace. This Core was initially developed over IS years ago and has consistently provided the highest quality services to over 100 Program Investigators and their collaborators at Washington University and around the world. The addition of the Cell Processing Core provides pulmonary investigators with the ability to acquire high yield, carefully phenotyped primary epithelial cell cultures from human and mouse lung specimens differentiated at the air-liquid interface, used for mechanistic in vitro studies. The Core serves two other large center grants (a SCCOR in COPD and a PPG in Lung Remodeling) enabling considerable cost savings and efficient utilization of personnel with specialized skills in a high throughput environment.

Public Health Relevance

The study of asthma pathogenesis in human and mouse models relies extensively on histological analyses and the use of primary epithelial cell cultures from asthmatics and normal controls and mice with varied genetic backgrounds in experimental models of asthma. The Tissue and Cell Processing Core facilitates these studies for Program investigators, providing expertise and techniques that each Project could not acquire in a cost-efficient manner. Centralized, standardized Tissue and Cell Processing also provides and consistent quality across Projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI070489-07
Application #
8378545
Study Section
Special Emphasis Panel (ZAI1-PA-I)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
7
Fiscal Year
2012
Total Cost
$163,785
Indirect Cost
$56,032
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Keeler, Shamus P; Agapov, Eugene V; Hinojosa, Michael E et al. (2018) Influenza A Virus Infection Causes Chronic Lung Disease Linked to Sites of Active Viral RNA Remnants. J Immunol 201:2354-2368
Myung, Jihwan; Schmal, Christoph; Hong, Sungho et al. (2018) The choroid plexus is an important circadian clock component. Nat Commun 9:1062
Liu, Yongjian; Gunsten, Sean P; Sultan, Deborah H et al. (2017) PET-based Imaging of Chemokine Receptor 2 in Experimental and Disease-related Lung Inflammation. Radiology 283:758-768
Steed, Ashley L; Christophi, George P; Kaiko, Gerard E et al. (2017) The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science 357:498-502
Woodruff, Prescott G; van den Berge, Maarten; Boucher, Richard C et al. (2017) American Thoracic Society/National Heart, Lung, and Blood Institute Asthma-Chronic Obstructive Pulmonary Disease Overlap Workshop Report. Am J Respir Crit Care Med 196:375-381
Chatterjee, Srirupa; Luthra, Priya; Esaulova, Ekaterina et al. (2017) Structural basis for human respiratory syncytial virus NS1-mediated modulation of host responses. Nat Microbiol 2:17101
Li, Shuai; O'Neill, Sofia R S; Zhang, Yong et al. (2017) Estrogen receptor ? is required for oviductal transport of embryos. FASEB J 31:1595-1607
Benedetto, Roberta; Ousingsawat, Jiraporn; Wanitchakool, Podchanart et al. (2017) Epithelial Chloride Transport by CFTR Requires TMEM16A. Sci Rep 7:12397
Schobel, Seth A; Stucker, Karla M; Moore, Martin L et al. (2016) Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene. Sci Rep 6:26311
Currier, Michael G; Lee, Sujin; Stobart, Christopher C et al. (2016) EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622

Showing the most recent 10 out of 84 publications