A key component in the systems biology approach to the study of asthmatic disease is the profiling of transcriptome and immune mediators. Over the last decade, our group has published on the utilization of various cDNA profiling technology including microarrays from commercial sources, such as Affymetrix and Agilent, and technology developed custom to the lab, such as representational difference analysis, differential display analysis, and serial analysis of gene expression (SAGE). Recently due to our need to include several novel genes that we found which are not represented on any commercial arrays, we have established our own custom arrays focusing on genes important in immune and oxidative responses. In addition, we have also investigated several gene network- pathway programs to support data analysis. The main goal of this core is to extend our expertise to investigators in this U19 Project, and to provide a userfriendly, one-stop facility for the broad analysis of cDNA and protein arrays relevant to immunity and oxidative stress, two issues of prominence in this proposal. The goals are: 1. To provide validated, sensitive and standardized human and mouse cDNA profiling technology for U19 investigators. 2. To provide help in the usage of broad human and mouse cytokine/chemokine and immune protein arrays. 3. To assist in the bioinformatics analysis of data from the cDNA and protein arrays;the Core will provide access and assistance in using computer programs to analyze the data, and pathway programs to assist in the interpretation of data to understand its biological significance.
| Hua, Xiaoyang; Naselsky, Warren C; Jania, Corey M et al. (2016) Mast Cell Deficiency Limits the Development of Chronic Rhinosinusitis in Mice. Ann Otol Rhinol Laryngol 125:290-6 |
| Kang, Min-Jong; Yoon, Chang Min; Kim, Bo Hye et al. (2015) Suppression of NLRX1 in chronic obstructive pulmonary disease. J Clin Invest 125:2458-62 |
| Aleman, Maria M; Mills, Katherine; Almond, Martha et al. (2015) Reply: To PMID 25195169. J Allergy Clin Immunol 135:836-7 |
| Wu, Weidong; Wages, Phillip A; Devlin, Robert B et al. (2015) SRC-mediated EGF receptor activation regulates ozone-induced interleukin 8 expression in human bronchial epithelial cells. Environ Health Perspect 123:231-6 |
| Hernandez, Michelle L; Mills, Katherine; Almond, Martha et al. (2015) IL-1 receptor antagonist reduces endotoxin-induced airway inflammation in healthy volunteers. J Allergy Clin Immunol 135:379-85 |
| Aleman, Maria M; Kesic, Matthew J; Mills, Katherine H et al. (2015) The IL-1 axis is associated with airway inflammation after O3 exposure in allergic asthmatic patients. J Allergy Clin Immunol 136:1099-101.e2 |
| Bennett, William D; Alexis, Neil E; Almond, Martha et al. (2014) Effect of inhaled endotoxin on mucociliary clearance and airway inflammation in mild smokers and nonsmokers. J Aerosol Med Pulm Drug Deliv 27:459-65 |
| Wu, Weidong; Muller, Robin; Berhane, Kiros et al. (2014) Inflammatory response of monocytes to ambient particles varies by highway proximity. Am J Respir Cell Mol Biol 51:802-9 |
| Fry, Rebecca C; Rager, Julia E; Bauer, Rebecca et al. (2014) Air toxics and epigenetic effects: ozone altered microRNAs in the sputum of human subjects. Am J Physiol Lung Cell Mol Physiol 306:L1129-37 |
| Brickey, Willie June (2013) Expression profiling to identify candidate genes associated with allergic phenotypes. Methods Mol Biol 1032:287-95 |
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