Inthisrenewalapplication,theScientificCore(CoreB)willcontinuetobuilduponthestrengthsofthe HumanImmuneMonitoringCenter(HIMC)andtheassaysdevelopedduringtheinitialgrantperiod.The HIMCisanidealenvironmentforstandardization,technologydevelopment,anddataanalysis,andhasbuilt anarrayofhigh-throughputandhigh-contentassaysthatareunparalleledinasinglecenterandinstitution. Overtheinitialfundingperiod,theScientificCoreaddedtothismenuoftechnologieswithuniqueCyTOF panelsandcombinedTCRseq/geneexpressionassaysatthesingle-celllevel.Overtheproposednext fundingperiod,CoreBwillcontinuetoprovidestandardizedimmunologicalassaysacrossstudieswithinthe U19project,focusingonsingle-cellassaysofallergen-responsivecellsandglobalimmuneprofiling.Itwill alsocontinuetodevelopnovelapproachesandworkwiththeindividualprojectstominetheimmunological datagenerated.
The specificaims are: 1.Toprovidestandardizedimmunemonitoringassaysacrossprojects.Thesewillincludedetailed immunophenotypingofwholebloodandPBMCsbymasscytometry(CyTOF),includingabasophil-centric CyTOFpaneldevelopedinthelabofSeanBendall(Co-InvestigatorofCoreB).TheCorewillalsooffer62- plexLuminexcytokinearraystoassessserumandcellsupernatantsforTh1/Th2/Th17cytokinesandsingle- cellTCRseq+geneexpressionforallergen-specificTcells.Finally,theCorewillperformMultiplexedIon BeamImaging(MIBI)ofesophageal,gastricandduodenalbiopsyspecimens,inthelabofRobertAngelo (Co-InvestigatorofCoreB).Byusingthesamestandardizedassaysacrossallcohorts,wewillgreatly increasethepowertotesthypothesesrelatedtoanyofthereadoutsoftheseassays. 2.Todevelopnovelimmunemonitoringassayswithindividualprojects.TheCyTOF,MIBI,andTCRseq panelswillcontinuetobedevelopedbasedonnewknowledgegeneratedwithintheprojects,aswellaskey observationsmadeintheallergyfieldgenerally.Wealsoplantoofferanewimmunoassayplatform, SomaScanfromSomaLogic,for1300solubleproteinanalytes,asanadditionaldiscoverytool. 3.Tofacilitateminingofimmunemonitoringdata.UsingStanfordDataMiner(SDM),theCorewillwork withtheDataManagementandAnalysisCoreCtominedatausingbioinformaticstools.SDMwillprovidea structuredframeworkforextractinglaboratorydatalinkedtoclinicalanddemographicvariables,facilitating dataminingandreducingthepossibilityoferrors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI104209-07
Application #
9851791
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2020-02-01
Budget End
2021-01-31
Support Year
7
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Tsai, Cheng-Ting; Mukai, Kaori; Robinson, Peter V et al. (2018) Isotype-specific agglutination-PCR (ISAP): A sensitive and multiplex method for measuring allergen-specific IgE. J Allergy Clin Immunol 141:1901-1904.e15
Gaudenzio, Nicolas; Marichal, Thomas; Galli, Stephen J et al. (2018) Genetic and Imaging Approaches Reveal Pro-Inflammatory and Immunoregulatory Roles of Mast Cells in Contact Hypersensitivity. Front Immunol 9:1275
Purington, Natasha; Chinthrajah, R Sharon; Long, Andrew et al. (2018) Eliciting Dose and Safety Outcomes From a Large Dataset of Standardized Multiple Food Challenges. Front Immunol 9:2057
Hartmann, Felix J; Simonds, Erin F; Bendall, Sean C (2018) A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis. Sci Rep 8:10770
Klein, Ofir; Roded, Amit; Hirschberg, Koret et al. (2018) Imaging FITC-dextran as a Reporter for Regulated Exocytosis. J Vis Exp :
Zhang, Wenming; Lin, Chunrong; Sampath, Vanitha et al. (2018) Impact of allergen immunotherapy in allergic asthma. Immunotherapy 10:579-593
Sampath, Vanitha; Sindher, Sayantani B; Zhang, Wenming et al. (2018) New treatment directions in food allergy. Ann Allergy Asthma Immunol 120:254-262
Sindher, Sayantani B; Long, Andrew; Acharya, Swati et al. (2018) The Use of Biomarkers to Predict Aero-Allergen and Food Immunotherapy Responses. Clin Rev Allergy Immunol 55:190-204
Mukai, Kaori; Tsai, Mindy; Saito, Hirohisa et al. (2018) Mast cells as sources of cytokines, chemokines, and growth factors. Immunol Rev 282:121-150
Keren, Leeat; Bosse, Marc; Marquez, Diana et al. (2018) A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging. Cell 174:1373-1387.e19

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