The mission of this HIPC program is to use hypothesis free approaches to define comprehensive molecular profiles of T cell subsets in health and disease (DENV and MTB). The areas of interest cover an extremely wide spectrum of ?omics? analysis including: (i) analysis of global gene expression, (ii) changes in global epigenetics profiles (genome-wide enhancer profiles), and (iii) defining single cell gene expression profile in protective T cell memory subsets from MTB and DENV infected/vaccinated subjects. All these ?omics? applications rely on standardized and well-validated high-throughput next-generation sequencing methods ? a key service provided by this Core. The HIPC-Sequencing core will support all next-generation sequencing activities for all the three projects involved in this program. This will be facilitated by the extensive genomics expertise of its personnel and the availability of cutting-edge technology and automated platforms to generate highly reproducible and robust sequencing data from a large number of samples. Practically, the core will interface directly with projects 1, 2 and 3 and provide a standardized and quality controlled pipeline consisting of receiving samples, sorting cells, preparing RNA/DNA, high throughput next-generation sequencing and transferring the resulting data to the Data Management Core for subsequent analysis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI118626-01
Application #
8931823
Study Section
Special Emphasis Panel (ZAI1-LGR-I (M1))
Project Start
Project End
Budget Start
2015-06-15
Budget End
2016-05-31
Support Year
1
Fiscal Year
2015
Total Cost
$250,466
Indirect Cost
$90,330
Name
La Jolla Institute
Department
Type
DUNS #
603880287
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Schulten, Véronique; Tripple, Victoria; Seumois, Grégory et al. (2018) Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells. J Allergy Clin Immunol 141:775-777.e6
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