Viralhemorrhagicfevers,ofwhichEbolavirus(EBOV)andLassavirus(LASV)areprototypicalexamples,area major source of human su?ering. Recent outbreaks have raised concerns about the possibility of a global outbreak.TheultimategoaloftheConsortiumforViralSystemsBiology(CViSB)istode?nenetworksofhost and viral factors that drive clinical outcomes in Ebola virus disease (EVD) and Lassa fever (LF). The responsibilities and contributions of the Technology Core fall into three main categories: data generation, technology development and standardization, and evaluation and incorporation of emerging technologies. Althoughthesecategoriesaredistinct,theyaretightlyintegrated.TheCore?sroleasdatagenerationhubfor CViSB also mandates that we continually work to develop and improve our methods and to rapidly incorporatenoveltechnologicaladvancestoensurethatweremainastate-of-the-art,productivememberof the Center. The Technology Core?s Aims are focused onthedevelopment,adaptationandstandardizationofinnovative experimental approaches in four main areas: (1) deep characterization of theadaptiveimmuneresponseto infection, (2) functional pro?ling of antiviral serum activity, (3) isolation and characterization of protective monoclonalantibodiesand(4)cost-e?cientviralgenomesequencing.InadditiontosupportingtheCenter?s ProjectsandcontributingtotheoverallsuccessofCViSB,theTechnologyCorewilltranslateits technicaland methodological advances into standardized, reproducible protocols that will bene?t other Centers and the broader infectious disease community.
|Suchard, Marc A; Lemey, Philippe; Baele, Guy et al. (2018) Bayesian phylogenetic and phylodynamic data integration using BEAST 1.10. Virus Evol 4:vey016|
|Gunn, Bronwyn M; Yu, Wen-Han; Karim, Marcus M et al. (2018) A Role for Fc Function in Therapeutic Monoclonal Antibody-Mediated Protection against Ebola Virus. Cell Host Microbe 24:221-233.e5|
|Saphire, Erica Ollmann; Schendel, Sharon L; Gunn, Bronwyn M et al. (2018) Antibody-mediated protection against Ebola virus. Nat Immunol 19:1169-1178|
|Rambaut, Andrew; Drummond, Alexei J; Xie, Dong et al. (2018) Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7. Syst Biol 67:901-904|
|Siddle, Katherine J; Eromon, Philomena; Barnes, Kayla G et al. (2018) Genomic Analysis of Lassa Virus during an Increase in Cases in Nigeria in 2018. N Engl J Med 379:1745-1753|
|Saphire, Erica Ollmann; Schendel, Sharon L; Fusco, Marnie L et al. (2018) Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection. Cell 174:938-952.e13|
|Holmes, Edward C; Rambaut, Andrew; Andersen, Kristian G (2018) Pandemics: spend on surveillance, not prediction. Nature 558:180-182|