Core - Preparation, Storage, and Shipping of Plant Extracts and Assays

Hecht, Sidney Michael

Abstract

This Core facility will be responsible for carrying out the preparation, storage and shipping of plant extracts, and much of the assay work run within the NPDDG. This is to include . soaking of plant materials; weighing, packaging, shipping and inventorying of plant extracts . testing of extracts for the presence of DNA damaging agents, DNA topoisomerase inhibitors and DNA polymerase beta inhibitors, in support of Programs 2. . isolation of purified DNA topoisomerase I and DNA polymerase beta for assay work . screening of samples received from NCI natural products repository . guiding the choice of extracts by the development/application of secondary discrimination assays in support of Laboratory Programs 2 . guiding the fractionation of extracts within Laboratory Program 3

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Program--Cooperative Agreements (U19)
• Project #: 5U19CA050771-07
• Application #: 5207612
• Support Year: 7
• Fiscal Year: 1996

Publications

Cao, Shugeng; Murphy, Brian T; Foster, Caleb et al. (2009) Bioactivities of simplified adociaquinone B and naphthoquinone derivatives against Cdc25B, MKP-1, and MKP-3 phosphatases. Bioorg Med Chem 17:2276-81

Clement, Jason A; Li, Mei; Hecht, Sidney M et al. (2006) Bioactive isomalabaricane triterpenoids from Rhabdastrella globostellata that stabilize the binding of DNA polymerase beta to DNA. J Nat Prod 69:373-6

Cao, Shugeng; Foster, Caleb; Brisson, Marni et al. (2005) Halenaquinone and xestoquinone derivatives, inhibitors of Cdc25B phosphatase from a Xestospongia sp. Bioorg Med Chem 13:999-1003

Cao, Shugeng; Foster, Caleb; Lazo, John S et al. (2005) Four diterpenoid inhibitors of Cdc25B phosphatase from a marine anemone. Bioorg Med Chem 13:5830-4

Chaturvedula, V S Prakash; Gao, Zhijie; Jones, Shannon H et al. (2004) A new ursane triterpene from Monochaetum vulcanicum that inhibits DNA polymerase beta lyase. J Nat Prod 67:899-901

Chaturvedula, V S Prakash; Zhou, Bing-Nan; Gao, Zhijie et al. (2004) New lupane triterpenoids from Solidago canadensis that inhibit the lyase activity of DNA polymerase beta. Bioorg Med Chem 12:6271-5

Prakash Chaturvedula, V S; Hecht, Sidney M; Gao, Zhijie et al. (2004) New neolignans that inhibit DNA polymerase beta lyase. J Nat Prod 67:964-7

Prakash Chaturvedula, V S; Schilling, Jennifer K; Johnson, Randall K et al. (2003) New cytotoxic lupane triterpenoids from the twigs of Coussarea paniculata. J Nat Prod 66:419-22

Prakash Chaturvedula, V S; Gao, Zhijie; Hecht, Sidney M et al. (2003) A new acylated oleanane triterpenoid from Couepia polyandra that inhibits the lyase activity of DNA polymerase beta. J Nat Prod 66:1463-5

Zhou, B N; Johnson, R K; Mattern, M R et al. (2001) The first naturally occurring Tie2 kinase inhibitor. Org Lett 3:4047-9